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- W4251170034 abstract "F8dl is a simian virus 40 early-region deletion mutant that lacks the sequences between 0.169 and 0.423 map units. We show that cloned F8dl DNA immortalized early-passage Fisher rat embryo cells with an efficiency that was about 20% of that of cloned wild-type simian virus 40 DNA. In contrast, we detected no immortalized colonies when we transfected the cells with DNA of five other early-region deletion mutants that do not make stable truncated forms of T antigen. Since all five of these mutants have intact early- and late-region control sequences, we conclude that these control sequences are not sufficient for immortalization. Three of the mutants that did not immortalize did make a normal small t antigen, suggesting that the expression of this protein alone is not sufficient for immortalization of early-passage Fisher rat embryo cells." @default.
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- W4251170034 date "1985-05-01" @default.
- W4251170034 modified "2023-09-30" @default.
- W4251170034 title "The Simian Virus 40 Sequences Between 0.169 and 0.423 Map Units Are Not Essential to Immortalize Early-Passage Rat Embryo Cells" @default.
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- W4251170034 doi "https://doi.org/10.1128/mcb.5.5.1191-1194.1985" @default.
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