Matches in SemOpenAlex for { <https://semopenalex.org/work/W4251174242> ?p ?o ?g. }
Showing items 1 to 75 of
75
with 100 items per page.
- W4251174242 endingPage "290" @default.
- W4251174242 startingPage "283" @default.
- W4251174242 abstract "Dipeptidyl peptidase II (DPP II; EC 3.4.14.2) from rat kidney was purified to a specific activity of 65.4µmol/min per mg of protein for Lys-Ala-β-naphthylamide. The N-terminal and partial amino acid sequences of the enzyme were determined. The peptide sequences were used to identify expressed sequence tag (EST) clones. By using the cDNA fragment of one of the EST clones as a probe, we isolated a cDNA clone with 1710bp encoding DPP II from a rat kidney cDNA library. The cDNA of rat DPP II contained an open reading frame of 1500bp, coding for a protein of 500 amino acids. The first 10 residues of the purified enzyme matched the deduced protein sequence starting with residue 37, suggesting the presence of a signal peptide. The mature enzyme (464 residues) had a calculated molecular mass of 51400Da, which was lower than the value (about 60000Da) determined by SDS/PAGE; and the deduced amino acid sequence showed six potential N-glycosylation sites. The deduced amino acid sequence of rat DPP II shared high similarity with quiescent-cell proline dipeptidase (78% identity) and prolyl carboxypeptidase (38% identity) and bore the putative catalytic triad (Ser, Asp, His) conserved in serine peptidase families. We transiently transfected COS-7 cells with pcDNA3.1 containing the cloned cDNA and obtained the overexpression of an immunoreactive protein (of about 60000Da). The transfected cells showed Lys-Ala-methylcoumarinamide-hydrolysing activity that was 50 times higher than the control cells." @default.
- W4251174242 created "2022-05-12" @default.
- W4251174242 creator A5014846725 @default.
- W4251174242 creator A5021620288 @default.
- W4251174242 creator A5027876079 @default.
- W4251174242 creator A5034152962 @default.
- W4251174242 creator A5049505930 @default.
- W4251174242 creator A5056380204 @default.
- W4251174242 creator A5057559424 @default.
- W4251174242 date "2001-01-08" @default.
- W4251174242 modified "2023-09-23" @default.
- W4251174242 title "Cloning and functional expression of rat kidney dipeptidyl peptidase II" @default.
- W4251174242 doi "https://doi.org/10.1042/bj3530283" @default.
- W4251174242 hasPublicationYear "2001" @default.
- W4251174242 type Work @default.
- W4251174242 citedByCount "14" @default.
- W4251174242 countsByYear W42511742422015 @default.
- W4251174242 countsByYear W42511742422016 @default.
- W4251174242 countsByYear W42511742422018 @default.
- W4251174242 crossrefType "journal-article" @default.
- W4251174242 hasAuthorship W4251174242A5014846725 @default.
- W4251174242 hasAuthorship W4251174242A5021620288 @default.
- W4251174242 hasAuthorship W4251174242A5027876079 @default.
- W4251174242 hasAuthorship W4251174242A5034152962 @default.
- W4251174242 hasAuthorship W4251174242A5049505930 @default.
- W4251174242 hasAuthorship W4251174242A5056380204 @default.
- W4251174242 hasAuthorship W4251174242A5057559424 @default.
- W4251174242 hasConcept C104317684 @default.
- W4251174242 hasConcept C145770059 @default.
- W4251174242 hasConcept C153911025 @default.
- W4251174242 hasConcept C167625842 @default.
- W4251174242 hasConcept C181199279 @default.
- W4251174242 hasConcept C187882448 @default.
- W4251174242 hasConcept C189482833 @default.
- W4251174242 hasConcept C19924922 @default.
- W4251174242 hasConcept C2776414213 @default.
- W4251174242 hasConcept C2781469919 @default.
- W4251174242 hasConcept C47289529 @default.
- W4251174242 hasConcept C515207424 @default.
- W4251174242 hasConcept C55493867 @default.
- W4251174242 hasConcept C86803240 @default.
- W4251174242 hasConceptScore W4251174242C104317684 @default.
- W4251174242 hasConceptScore W4251174242C145770059 @default.
- W4251174242 hasConceptScore W4251174242C153911025 @default.
- W4251174242 hasConceptScore W4251174242C167625842 @default.
- W4251174242 hasConceptScore W4251174242C181199279 @default.
- W4251174242 hasConceptScore W4251174242C187882448 @default.
- W4251174242 hasConceptScore W4251174242C189482833 @default.
- W4251174242 hasConceptScore W4251174242C19924922 @default.
- W4251174242 hasConceptScore W4251174242C2776414213 @default.
- W4251174242 hasConceptScore W4251174242C2781469919 @default.
- W4251174242 hasConceptScore W4251174242C47289529 @default.
- W4251174242 hasConceptScore W4251174242C515207424 @default.
- W4251174242 hasConceptScore W4251174242C55493867 @default.
- W4251174242 hasConceptScore W4251174242C86803240 @default.
- W4251174242 hasIssue "2" @default.
- W4251174242 hasLocation W42511742421 @default.
- W4251174242 hasOpenAccess W4251174242 @default.
- W4251174242 hasPrimaryLocation W42511742421 @default.
- W4251174242 hasRelatedWork W1988199343 @default.
- W4251174242 hasRelatedWork W1996545176 @default.
- W4251174242 hasRelatedWork W2004344796 @default.
- W4251174242 hasRelatedWork W2024587461 @default.
- W4251174242 hasRelatedWork W2065739243 @default.
- W4251174242 hasRelatedWork W2074111310 @default.
- W4251174242 hasRelatedWork W2079592196 @default.
- W4251174242 hasRelatedWork W2121437105 @default.
- W4251174242 hasRelatedWork W2141409053 @default.
- W4251174242 hasRelatedWork W2419545453 @default.
- W4251174242 hasVolume "353" @default.
- W4251174242 isParatext "false" @default.
- W4251174242 isRetracted "false" @default.
- W4251174242 workType "article" @default.