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- W4251222019 abstract "We have developed in vitro culture systems for both “grain” and “vegetable” species of the genus Amaranthus. Leaf discs and hypocotyl segments from 2- to 3-wk-old seedlings of A. hypochondriacus, A. cruentus, and A. tricolor were cultured in B5 and MS media supplemented with 2,4-dichlorophenoxyacetic acid, α-naphthaleneacetic acid, benzyladenine and zeatin in various combinations. Rapidly growing callus and abnormal roots formed on leaf discs of A. hypochondriacus and A. cruentus in the presence of 0.1-1.0 mg/l 2,4-D. At higher levels (1.0-10.0 mg/l) of 2,4-D, embryo-like structures arose from the surface and veins of the leaf discs. Shoots formed on hypocotyl-derived callus of both grain species in B5 medium + 0.1 mg/l NAA and 0.1-1.0 mg/l zeatin. Lower ratios of zeatin/NAA stimulated root formation from hypocotyl segments. Viable mesophyll protoplasts were isolated from primary leaves of all three species, with an enzyme solution of 1% Cellulysin and 0.05% Pectolyase Y-23, producing a yield of more than 106 protoplasts/g fresh weight." @default.
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- W4251222019 date "1982-08-01" @default.
- W4251222019 modified "2023-10-18" @default.
- W4251222019 title "In Vitro Culture of Grain and Vegetable Amaranths (Amaranthus spp.)" @default.
- W4251222019 doi "https://doi.org/10.2307/2443080" @default.
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