Matches in SemOpenAlex for { <https://semopenalex.org/work/W4251472946> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W4251472946 endingPage "359" @default.
- W4251472946 startingPage "359" @default.
- W4251472946 abstract "The inheritance of the apolipoprotein E (apoE) ϵ4 allele is a prevailing risk factor for sporadic and familial Alzheimer's disease (AD). ApoE isoforms bind directly to Alzheimer's amyloid β (Aβ) peptides both in vitro and in vivo. Recent studies suggest that association of apoE with lipids may modulate its interaction with Aβ. We examined the binding of lipid-associated and delipidated apoE3 and apoE4 isoforms to Aβ utilizing a solid-phase binding assay and estimated the dissociation constants for the interaction of various apoE and Aβ species. Using native apoE isoforms from stably transfected RAW 264 and human embryonic kidney 293 cells, apoE3 had greater affinity than apoE4 for both Aβ1-40 and Aβ1-42. Delipidation of apoE decreased its affinity for Aβ peptides by 5-10-fold and abolished the isoform-specificity. Conversely, incorporation of apoE isoforms produced by baculovirus-infected Sf9 cells into reconstituted human high-density-lipoprotein lipoparticles restored the affinity values for Aβ peptides and resulted in preferential binding of apoE3. The data demonstrate that native lipid-associated apoE3 binds to Aβ peptides with 2-3-fold higher affinity than lipid-associated apoE4. Since the isoforms' binding efficiency correlate inversely with the risk of developing late-onset AD, the results suggest a possible involvement of apoE3 in the clearance or routing out of Aβ from the central nervous system as one of the mechanisms underlying the pathology of the disease." @default.
- W4251472946 created "2022-05-12" @default.
- W4251472946 creator A5002512182 @default.
- W4251472946 creator A5014972559 @default.
- W4251472946 creator A5022510840 @default.
- W4251472946 creator A5025043670 @default.
- W4251472946 creator A5027745914 @default.
- W4251472946 creator A5036566811 @default.
- W4251472946 creator A5041419276 @default.
- W4251472946 creator A5050120025 @default.
- W4251472946 creator A5060394127 @default.
- W4251472946 creator A5067350278 @default.
- W4251472946 creator A5077022989 @default.
- W4251472946 creator A5081547926 @default.
- W4251472946 date "2000-06-01" @default.
- W4251472946 modified "2023-10-18" @default.
- W4251472946 title "Lipidation of apolipoprotein E influences its isoform-specific interaction with Alzheimer’s amyloid β peptides" @default.
- W4251472946 doi "https://doi.org/10.1042/0264-6021:3480359" @default.
- W4251472946 hasPublicationYear "2000" @default.
- W4251472946 type Work @default.
- W4251472946 citedByCount "62" @default.
- W4251472946 countsByYear W42514729462012 @default.
- W4251472946 countsByYear W42514729462013 @default.
- W4251472946 countsByYear W42514729462014 @default.
- W4251472946 countsByYear W42514729462015 @default.
- W4251472946 countsByYear W42514729462017 @default.
- W4251472946 countsByYear W42514729462018 @default.
- W4251472946 countsByYear W42514729462019 @default.
- W4251472946 countsByYear W42514729462022 @default.
- W4251472946 crossrefType "journal-article" @default.
- W4251472946 hasAuthorship W4251472946A5002512182 @default.
- W4251472946 hasAuthorship W4251472946A5014972559 @default.
- W4251472946 hasAuthorship W4251472946A5022510840 @default.
- W4251472946 hasAuthorship W4251472946A5025043670 @default.
- W4251472946 hasAuthorship W4251472946A5027745914 @default.
- W4251472946 hasAuthorship W4251472946A5036566811 @default.
- W4251472946 hasAuthorship W4251472946A5041419276 @default.
- W4251472946 hasAuthorship W4251472946A5050120025 @default.
- W4251472946 hasAuthorship W4251472946A5060394127 @default.
- W4251472946 hasAuthorship W4251472946A5067350278 @default.
- W4251472946 hasAuthorship W4251472946A5077022989 @default.
- W4251472946 hasAuthorship W4251472946A5081547926 @default.
- W4251472946 hasBestOaLocation W42514729462 @default.
- W4251472946 hasConcept C104317684 @default.
- W4251472946 hasConcept C126322002 @default.
- W4251472946 hasConcept C153911025 @default.
- W4251472946 hasConcept C185592680 @default.
- W4251472946 hasConcept C2778163477 @default.
- W4251472946 hasConcept C2779134260 @default.
- W4251472946 hasConcept C53345823 @default.
- W4251472946 hasConcept C55493867 @default.
- W4251472946 hasConcept C57089818 @default.
- W4251472946 hasConcept C62746215 @default.
- W4251472946 hasConcept C71924100 @default.
- W4251472946 hasConcept C86803240 @default.
- W4251472946 hasConceptScore W4251472946C104317684 @default.
- W4251472946 hasConceptScore W4251472946C126322002 @default.
- W4251472946 hasConceptScore W4251472946C153911025 @default.
- W4251472946 hasConceptScore W4251472946C185592680 @default.
- W4251472946 hasConceptScore W4251472946C2778163477 @default.
- W4251472946 hasConceptScore W4251472946C2779134260 @default.
- W4251472946 hasConceptScore W4251472946C53345823 @default.
- W4251472946 hasConceptScore W4251472946C55493867 @default.
- W4251472946 hasConceptScore W4251472946C57089818 @default.
- W4251472946 hasConceptScore W4251472946C62746215 @default.
- W4251472946 hasConceptScore W4251472946C71924100 @default.
- W4251472946 hasConceptScore W4251472946C86803240 @default.
- W4251472946 hasIssue "2" @default.
- W4251472946 hasLocation W42514729461 @default.
- W4251472946 hasLocation W42514729462 @default.
- W4251472946 hasOpenAccess W4251472946 @default.
- W4251472946 hasPrimaryLocation W42514729461 @default.
- W4251472946 hasRelatedWork W1972781517 @default.
- W4251472946 hasRelatedWork W1996812601 @default.
- W4251472946 hasRelatedWork W2006574397 @default.
- W4251472946 hasRelatedWork W2048584108 @default.
- W4251472946 hasRelatedWork W2077788775 @default.
- W4251472946 hasRelatedWork W2080478929 @default.
- W4251472946 hasRelatedWork W2087533793 @default.
- W4251472946 hasRelatedWork W2092939690 @default.
- W4251472946 hasRelatedWork W2170697078 @default.
- W4251472946 hasRelatedWork W2617783430 @default.
- W4251472946 hasVolume "348" @default.
- W4251472946 isParatext "false" @default.
- W4251472946 isRetracted "false" @default.
- W4251472946 workType "article" @default.