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- W4251583832 abstract "The method described here can be used to prepare RNA from a variety of eukaryotic tissues. The critical factor in isolating RNA from eukaryotic tissues is inactivating the endogenous RNase and preventing introduction of RNase from external sources. In general, protocols for making RNA from eukaryotic organisms involve lysing the cells in the presence of a strong denaturant and deproteinizing agent which inhibits RNase as well as strips the protein away from the RNA. In this protocol, the RNA is then separated from DNA and other impurities by selective precipitation in high salt." @default.
- W4251583832 created "2022-05-12" @default.
- W4251583832 date "1990-01-01" @default.
- W4251583832 modified "2023-09-25" @default.
- W4251583832 title "Phenol/ <scp>SDS</scp> Method for Plant <scp>RNA</scp> Preparation" @default.
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- W4251583832 doi "https://doi.org/10.1002/0471142727.mb0403s09" @default.
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