Matches in SemOpenAlex for { <https://semopenalex.org/work/W4251595350> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W4251595350 endingPage "57" @default.
- W4251595350 startingPage "49" @default.
- W4251595350 abstract "Glutathione S-transferases (GSTs) are known to catalyze conjugations by facilitating the nucleophilic attack of the sulfhydryl group of endogenous reduced glutathione on electrophilic centers of a vast range of xenobiotic compounds, including insecticides and acaricides. Elevated levels of GSTs in the two-spotted spider mite, Tetranychus urticae Koch, have recently been associated with resistance to acaricides such as abamectin [Pestic. Biochem. Physiol. 72 (2002) 111]. GSTs from acaricide susceptible and resistant strains of T. urticae were purified by glutathione–agarose affinity chromatography and characterized by their Michaelis–Menten kinetics towards artificial substrates, i.e., 1-chloro-2,4-dinitrobenzene and monochlorobimane. The inhibitory potential of azocyclotin, dicumarol, and plumbagin was low (IC50 values > 100 μM), whereas ethacrynic acid was much more effective, exhibiting an IC50 value of 4.5 μM. GST activity is highest in 2–4-day-old female adults and dropped considerably with progressing age. Furthermore, molecular characteristics were determined for the first time of a GST from T. urticae, such as molecular weight (SDS–PAGE) and N-terminal amino acid sequencing (Edman degradation). Glutathione–agarose affinity purified GST from T. urticae strain WI has a molecular weight of 22.1 kDa. N-terminal amino acid sequencing revealed a homogeneity of ≈50% to insect GSTs closely related to insect class I GSTs (similar to mammalian Delta class GSTs)." @default.
- W4251595350 created "2022-05-12" @default.
- W4251595350 creator A5005471018 @default.
- W4251595350 date "2004-06-01" @default.
- W4251595350 modified "2023-10-16" @default.
- W4251595350 title "Purification and partial characterization of a glutathione S-transferase from the two-spotted spider mite, Tetranychus urticae" @default.
- W4251595350 doi "https://doi.org/10.1016/s0048-3575(04)00039-2" @default.
- W4251595350 hasPublicationYear "2004" @default.
- W4251595350 type Work @default.
- W4251595350 citedByCount "0" @default.
- W4251595350 crossrefType "journal-article" @default.
- W4251595350 hasAuthorship W4251595350A5005471018 @default.
- W4251595350 hasConcept C161176658 @default.
- W4251595350 hasConcept C181199279 @default.
- W4251595350 hasConcept C22508944 @default.
- W4251595350 hasConcept C2776906779 @default.
- W4251595350 hasConcept C2776907368 @default.
- W4251595350 hasConcept C2779248279 @default.
- W4251595350 hasConcept C2781092779 @default.
- W4251595350 hasConcept C33070731 @default.
- W4251595350 hasConcept C538909803 @default.
- W4251595350 hasConcept C55493867 @default.
- W4251595350 hasConcept C59822182 @default.
- W4251595350 hasConcept C6557445 @default.
- W4251595350 hasConcept C74714110 @default.
- W4251595350 hasConcept C86803240 @default.
- W4251595350 hasConceptScore W4251595350C161176658 @default.
- W4251595350 hasConceptScore W4251595350C181199279 @default.
- W4251595350 hasConceptScore W4251595350C22508944 @default.
- W4251595350 hasConceptScore W4251595350C2776906779 @default.
- W4251595350 hasConceptScore W4251595350C2776907368 @default.
- W4251595350 hasConceptScore W4251595350C2779248279 @default.
- W4251595350 hasConceptScore W4251595350C2781092779 @default.
- W4251595350 hasConceptScore W4251595350C33070731 @default.
- W4251595350 hasConceptScore W4251595350C538909803 @default.
- W4251595350 hasConceptScore W4251595350C55493867 @default.
- W4251595350 hasConceptScore W4251595350C59822182 @default.
- W4251595350 hasConceptScore W4251595350C6557445 @default.
- W4251595350 hasConceptScore W4251595350C74714110 @default.
- W4251595350 hasConceptScore W4251595350C86803240 @default.
- W4251595350 hasIssue "2" @default.
- W4251595350 hasLocation W42515953501 @default.
- W4251595350 hasOpenAccess W4251595350 @default.
- W4251595350 hasPrimaryLocation W42515953501 @default.
- W4251595350 hasRelatedWork W1976952993 @default.
- W4251595350 hasRelatedWork W2063626539 @default.
- W4251595350 hasRelatedWork W2116808963 @default.
- W4251595350 hasRelatedWork W2792050028 @default.
- W4251595350 hasRelatedWork W2914805826 @default.
- W4251595350 hasRelatedWork W2953611295 @default.
- W4251595350 hasRelatedWork W2986438076 @default.
- W4251595350 hasRelatedWork W3157209022 @default.
- W4251595350 hasRelatedWork W4251595350 @default.
- W4251595350 hasRelatedWork W2330380533 @default.
- W4251595350 hasVolume "79" @default.
- W4251595350 isParatext "false" @default.
- W4251595350 isRetracted "false" @default.
- W4251595350 workType "article" @default.