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- W4252007694 abstract "Abstract Previously, we demonstrated an energy-dependent injury to cultured liver endothelial cells during cold incubation in University of Wisconsin (UW) solution. Here, the effects of Histidine-Tryptophan-Ketoglutarate (HTK) and Euro-Collins (EC) solutions on these cells were studied. In HTK solution, 83 %± 4 % of the cells had lost viability after 9 h of incubation at 4°C. The addition of cyanide (1 mM) to simulate hypoxic conditions protected the cells to the extent that only 9%±1 % of the cells lost viability over the same period; the addition of glucose (10 mM) led to increased cell injury. ATP levels were highest in the incubations with the most rapid loss of viability. In Krebs-Henseleit buffer and EC solution, in contrast, cell injury increased upon addition of cyanide; the addition of glucose to Krebs-Henseleit buffer decreased injury. We conclude that the injury to cultured liver endothelial cells during cold incubation in HTK solution is energy-dependent, as it is in UW solution, whereas cells behave differently in EC solution and Krebs-Henseleit buffer." @default.
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- W4252007694 date "1993-07-01" @default.
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- W4252007694 title "Injury to cultured liver endothelial cells during cold preservation: energy-dependent versus energy-deficiency injury" @default.
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- W4252007694 doi "https://doi.org/10.1111/j.1432-2277.1993.tb00651.x" @default.
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