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- W4252251597 abstract "Late in infection, transcription of the EIIa gene is initiated primarily at map unit 72 of the adenovirus genome. A cell-free nuclear extract system was used to determine sequence elements important for the function of this late promoter. In such a system, the transcriptional activity of a circular template was found to be much higher (5- to 10-fold) than that of a linear template. The effect of template topology appeared to be dependent on two distal upstream elements with 5' boundaries located near -265 to -223 and -147 to -133 (in relation to the initiation site), since deletions of these regions reduced transcription of the circular template, in a stepwise fashion, to a level similar to that observed with the linear template. Further deletions revealed an element in the -116 region that appeared to be more important for transcription of the circular template (10-fold reduction) than for transcription of the linear template (3-fold reduction). Lastly, deletion of the TACAAA sequence in the -29 region resulted in further reduction in transcription, indicating that this element functions as a promoter in vitro." @default.
- W4252251597 created "2022-05-12" @default.
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- W4252251597 date "1988-05-01" @default.
- W4252251597 modified "2023-09-26" @default.
- W4252251597 title "Delineation of DNA sequences that are important for in vitro transcription from the adenovirus EIIa late promoter" @default.
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- W4252251597 doi "https://doi.org/10.1128/mcb.8.5.1906-1914.1988" @default.
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