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- W4252471775 abstract "We developed a pentaplex real-time PCR assay including Yersinia pestis-specific genes ypo2088, caf1, and pst located on the chromosome, as well as on plasmids pMT1 and pPCP1, respectively. Yersinia pseudotuberculosis-specific chromosomal gene opgG and the 18S ribosomal RNA gene served as an internal control for flea DNA. All targets showed 100% specificity and high sensitivity with limits of detection ranging from 1 to 100 femtograms. Using the assay, Y. pestis was detected in field-collected fleas and strains were differentiated 100% by their known plasmid profiles." @default.
- W4252471775 created "2022-05-12" @default.
- W4252471775 date "2020-10-01" @default.
- W4252471775 modified "2023-09-26" @default.
- W4252471775 doi "https://doi.org/10.1002/mbo3.v9.10" @default.
- W4252471775 hasPublicationYear "2020" @default.
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