Matches in SemOpenAlex for { <https://semopenalex.org/work/W4252617126> ?p ?o ?g. }
- W4252617126 endingPage "9466" @default.
- W4252617126 startingPage "9461" @default.
- W4252617126 abstract "An optical ruler based on ultrahigh-resolution colocalization of single fluorescent probes is described in this paper. It relies on the use of two unique families of fluorophores, namely energy-transfer fluorescent beads (TransFluoSpheres) and semiconductor nanocrystal quantum dots, that can be excited by a single laser wavelength but emit at different wavelengths. A multicolor sample-scanning confocal microscope was constructed that allows one to image each fluorescent light emitter, free of chromatic aberrations, by scanning the sample with nanometer scale steps with a piezo-scanner. The resulting spots are accurately localized by fitting them to the known shape of the excitation point-spread function of the microscope. We present results of two-dimensional colocalization of TransFluoSpheres (40 nm in diameter) and of nanocrystals (3–10 nm in diameter) and demonstrate distance-measurement accuracy of better than 10 nm using conventional far-field optics. This ruler bridges the gap between fluorescence resonance energy transfer, near- and far-field imaging, spanning a range of a few nanometers to tens of micrometers." @default.
- W4252617126 created "2022-05-12" @default.
- W4252617126 creator A5009848674 @default.
- W4252617126 creator A5025209661 @default.
- W4252617126 creator A5037701220 @default.
- W4252617126 creator A5041130874 @default.
- W4252617126 creator A5060214768 @default.
- W4252617126 creator A5060401840 @default.
- W4252617126 date "2000-08-08" @default.
- W4252617126 modified "2023-10-16" @default.
- W4252617126 title "Ultrahigh-resolution multicolor colocalization of single fluorescent probes" @default.
- W4252617126 cites W1965238080 @default.
- W4252617126 cites W1978249718 @default.
- W4252617126 cites W1994065888 @default.
- W4252617126 cites W1995413508 @default.
- W4252617126 cites W1995945562 @default.
- W4252617126 cites W1998948607 @default.
- W4252617126 cites W2008679154 @default.
- W4252617126 cites W2017038627 @default.
- W4252617126 cites W2017355570 @default.
- W4252617126 cites W2018983175 @default.
- W4252617126 cites W2021179200 @default.
- W4252617126 cites W2021748684 @default.
- W4252617126 cites W2028989854 @default.
- W4252617126 cites W2031740917 @default.
- W4252617126 cites W2039501915 @default.
- W4252617126 cites W2039655645 @default.
- W4252617126 cites W2040431881 @default.
- W4252617126 cites W2042050610 @default.
- W4252617126 cites W2045930926 @default.
- W4252617126 cites W2058469330 @default.
- W4252617126 cites W2062485439 @default.
- W4252617126 cites W2071317718 @default.
- W4252617126 cites W2072113905 @default.
- W4252617126 cites W2089726895 @default.
- W4252617126 cites W2090897377 @default.
- W4252617126 cites W2094437628 @default.
- W4252617126 cites W2098001162 @default.
- W4252617126 cites W2108691381 @default.
- W4252617126 cites W2123605247 @default.
- W4252617126 cites W2141802984 @default.
- W4252617126 cites W2145055214 @default.
- W4252617126 cites W2153559476 @default.
- W4252617126 cites W2155919146 @default.
- W4252617126 cites W2156310052 @default.
- W4252617126 cites W2166082940 @default.
- W4252617126 cites W2170508213 @default.
- W4252617126 cites W2181352030 @default.
- W4252617126 cites W4237348051 @default.
- W4252617126 doi "https://doi.org/10.1073/pnas.170286097" @default.
- W4252617126 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10931959" @default.
- W4252617126 hasPublicationYear "2000" @default.
- W4252617126 type Work @default.
- W4252617126 citedByCount "260" @default.
- W4252617126 countsByYear W42526171262012 @default.
- W4252617126 countsByYear W42526171262013 @default.
- W4252617126 countsByYear W42526171262014 @default.
- W4252617126 countsByYear W42526171262015 @default.
- W4252617126 countsByYear W42526171262016 @default.
- W4252617126 countsByYear W42526171262017 @default.
- W4252617126 countsByYear W42526171262018 @default.
- W4252617126 countsByYear W42526171262020 @default.
- W4252617126 countsByYear W42526171262021 @default.
- W4252617126 countsByYear W42526171262022 @default.
- W4252617126 countsByYear W42526171262023 @default.
- W4252617126 crossrefType "journal-article" @default.
- W4252617126 hasAuthorship W4252617126A5009848674 @default.
- W4252617126 hasAuthorship W4252617126A5025209661 @default.
- W4252617126 hasAuthorship W4252617126A5037701220 @default.
- W4252617126 hasAuthorship W4252617126A5041130874 @default.
- W4252617126 hasAuthorship W4252617126A5060214768 @default.
- W4252617126 hasAuthorship W4252617126A5060401840 @default.
- W4252617126 hasBestOaLocation W42526171261 @default.
- W4252617126 hasConcept C120665830 @default.
- W4252617126 hasConcept C121332964 @default.
- W4252617126 hasConcept C136009344 @default.
- W4252617126 hasConcept C138268822 @default.
- W4252617126 hasConcept C147080431 @default.
- W4252617126 hasConcept C154945302 @default.
- W4252617126 hasConcept C192562407 @default.
- W4252617126 hasConcept C40209533 @default.
- W4252617126 hasConcept C41008148 @default.
- W4252617126 hasConcept C49040817 @default.
- W4252617126 hasConcept C520434653 @default.
- W4252617126 hasConcept C6260449 @default.
- W4252617126 hasConcept C67649825 @default.
- W4252617126 hasConcept C86803240 @default.
- W4252617126 hasConcept C91881484 @default.
- W4252617126 hasConcept C95444343 @default.
- W4252617126 hasConceptScore W4252617126C120665830 @default.
- W4252617126 hasConceptScore W4252617126C121332964 @default.
- W4252617126 hasConceptScore W4252617126C136009344 @default.
- W4252617126 hasConceptScore W4252617126C138268822 @default.
- W4252617126 hasConceptScore W4252617126C147080431 @default.
- W4252617126 hasConceptScore W4252617126C154945302 @default.
- W4252617126 hasConceptScore W4252617126C192562407 @default.
- W4252617126 hasConceptScore W4252617126C40209533 @default.
- W4252617126 hasConceptScore W4252617126C41008148 @default.