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- W4252802020 abstract "A procedure for extracting RNA-free plasmid DNA from bacterial cells is described. The method is simple and rapid enough to obtain pure plasmid DNA in 8 to 10 h after plasmid amplification. The protocol uses the alkaline extraction procedure described by Birnboim and Doly (1979, Nucl. Acid Res. 7, 1513–1523). Plasmid DNA is then separated from high-molecular-weight RNA by ammonium acetate precipitation and from low-molecular-weight RNA contaminants by Ultrogel A2 column chromatography. The plasmid DNA obtained by this inexpensive technique is sufficiently pure to be used for restriction endonuclease analysis, 5′-end labeling, S1 mapping, DNA sequencing, and colony hydridization." @default.
- W4252802020 created "2022-05-12" @default.
- W4252802020 date "1985-01-01" @default.
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- W4252802020 title "Isolation of specific chromosomes" @default.
- W4252802020 doi "https://doi.org/10.1016/0168-9525(85)90063-0" @default.
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