Matches in SemOpenAlex for { <https://semopenalex.org/work/W4252967976> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W4252967976 endingPage "56" @default.
- W4252967976 startingPage "49" @default.
- W4252967976 abstract "Caspase-9 is the apex caspase of the mitochondrial pathway of apoptosis, which plays a critical role in apoptotic initiation and progression. However, gene regulation of caspase-9 is largely unknown. This is in part due to the lack of information on the gene promoter. Here we have cloned the full-length cDNA of rat caspase-9 and have isolated promoter regions of this gene. The rat caspase-9 cDNA of 2058bp predicts a protein of 454 amino acids, which contains a caspase-recruitment domain (‘CARD’) at the N-terminus and enzymic domains at the C-terminus. The enzyme's active site, with a characteristic motif of QACGG, was also identified. Overall, rat and human caspase-9 have 71% identity. With the cDNA sequence, we subsequently isolated the proximal 5′-flanking regions of rat caspase-9 by the procedure of genomic walking. The 2270bp genomic segment is ‘TATA-less’, but contains several GC boxes. Elements binding known transcription factors such as Sp-1, Pit-1, CCAAT-enhancer-binding protein (C/EBP), glucocorticoid receptor and hypoxia-inducible factor 1 (HIF-1) were also identified. When cloned into reporter gene vectors, the genomic segment showed significant promoter activity, indicating that the 5′-flanking regions isolated by genomic walking contain the gene promoter of rat caspase-9. Of significance is that the cloned promoter segments were activated by severe hypoxia, conditions inducing caspase-9 transcription. Thus, the genomic sequences reported here contain not only the basal promoter of rat caspase-9 but also regulatory elements responsive to pathophysiological stimuli including hypoxia." @default.
- W4252967976 created "2022-05-12" @default.
- W4252967976 creator A5023791678 @default.
- W4252967976 creator A5039956551 @default.
- W4252967976 creator A5053799287 @default.
- W4252967976 creator A5084595032 @default.
- W4252967976 date "2001-11-08" @default.
- W4252967976 modified "2023-10-05" @default.
- W4252967976 title "cDNA cloning and promoter analysis of rat caspase-9" @default.
- W4252967976 doi "https://doi.org/10.1042/bj3600049" @default.
- W4252967976 hasPublicationYear "2001" @default.
- W4252967976 type Work @default.
- W4252967976 citedByCount "14" @default.
- W4252967976 countsByYear W42529679762014 @default.
- W4252967976 crossrefType "journal-article" @default.
- W4252967976 hasAuthorship W4252967976A5023791678 @default.
- W4252967976 hasAuthorship W4252967976A5039956551 @default.
- W4252967976 hasAuthorship W4252967976A5053799287 @default.
- W4252967976 hasAuthorship W4252967976A5084595032 @default.
- W4252967976 hasConcept C101762097 @default.
- W4252967976 hasConcept C104317684 @default.
- W4252967976 hasConcept C111936080 @default.
- W4252967976 hasConcept C150194340 @default.
- W4252967976 hasConcept C153911025 @default.
- W4252967976 hasConcept C187882448 @default.
- W4252967976 hasConcept C190283241 @default.
- W4252967976 hasConcept C31573885 @default.
- W4252967976 hasConcept C54355233 @default.
- W4252967976 hasConcept C65888428 @default.
- W4252967976 hasConcept C86339819 @default.
- W4252967976 hasConcept C86803240 @default.
- W4252967976 hasConcept C98424977 @default.
- W4252967976 hasConceptScore W4252967976C101762097 @default.
- W4252967976 hasConceptScore W4252967976C104317684 @default.
- W4252967976 hasConceptScore W4252967976C111936080 @default.
- W4252967976 hasConceptScore W4252967976C150194340 @default.
- W4252967976 hasConceptScore W4252967976C153911025 @default.
- W4252967976 hasConceptScore W4252967976C187882448 @default.
- W4252967976 hasConceptScore W4252967976C190283241 @default.
- W4252967976 hasConceptScore W4252967976C31573885 @default.
- W4252967976 hasConceptScore W4252967976C54355233 @default.
- W4252967976 hasConceptScore W4252967976C65888428 @default.
- W4252967976 hasConceptScore W4252967976C86339819 @default.
- W4252967976 hasConceptScore W4252967976C86803240 @default.
- W4252967976 hasConceptScore W4252967976C98424977 @default.
- W4252967976 hasIssue "1" @default.
- W4252967976 hasLocation W42529679761 @default.
- W4252967976 hasOpenAccess W4252967976 @default.
- W4252967976 hasPrimaryLocation W42529679761 @default.
- W4252967976 hasRelatedWork W10961269 @default.
- W4252967976 hasRelatedWork W11110039 @default.
- W4252967976 hasRelatedWork W11744232 @default.
- W4252967976 hasRelatedWork W1309511 @default.
- W4252967976 hasRelatedWork W24057152 @default.
- W4252967976 hasRelatedWork W25692858 @default.
- W4252967976 hasRelatedWork W34520794 @default.
- W4252967976 hasRelatedWork W40546165 @default.
- W4252967976 hasRelatedWork W46689033 @default.
- W4252967976 hasRelatedWork W50782919 @default.
- W4252967976 hasVolume "360" @default.
- W4252967976 isParatext "false" @default.
- W4252967976 isRetracted "false" @default.
- W4252967976 workType "article" @default.