Matches in SemOpenAlex for { <https://semopenalex.org/work/W4253000629> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W4253000629 endingPage "2826" @default.
- W4253000629 startingPage "2821" @default.
- W4253000629 abstract "Intracellular pullulanase was entirely extracted with sodium dodecylsulfate from the cells and was purified by means of ammonium sulfate fractionation and DEAE-cellulose and Sephadex chromatography. Crystalline pullulanase was precipitated with saturated ammonium sulfate solution. Intracellular pullulanase was purified over 150 fold in 17% yield to a final specific activity of 7000 per mg protein from the enzyme solution obtained by SDS-extraction. On ultracentrifugation analysis, the enzyme showed a symmetrical peak. The sedimentation coefficient, s20, w was 6.29 S. Polyacrylamide disc electrophoresis gave a main band and a sub-band, and both showed activity. Molecular weight of intracellular pullulanase was estimated to be (8±1) × 10,000 from gel filtration with Sephadex G-200 and to be (9±1) × 10,000 from sedimentation equilibrium. These values were higher than that (6~7 × 10,000) of extracellular pullulanase. Both enzymes differed slightly in thermal- and pH-stabilities." @default.
- W4253000629 created "2022-05-12" @default.
- W4253000629 creator A5042961127 @default.
- W4253000629 creator A5090369619 @default.
- W4253000629 date "1973-12-01" @default.
- W4253000629 modified "2023-09-27" @default.
- W4253000629 title "Purification, Crystallization and Some Properties of Intracellular Pullulanase from<i>Aerobacter aerogenes</i>" @default.
- W4253000629 doi "https://doi.org/10.1080/00021369.1973.10861090" @default.
- W4253000629 hasPublicationYear "1973" @default.
- W4253000629 type Work @default.
- W4253000629 citedByCount "13" @default.
- W4253000629 countsByYear W42530006292012 @default.
- W4253000629 countsByYear W42530006292018 @default.
- W4253000629 countsByYear W42530006292023 @default.
- W4253000629 crossrefType "journal-article" @default.
- W4253000629 hasAuthorship W4253000629A5042961127 @default.
- W4253000629 hasAuthorship W4253000629A5090369619 @default.
- W4253000629 hasConcept C101660505 @default.
- W4253000629 hasConcept C1388644 @default.
- W4253000629 hasConcept C163994747 @default.
- W4253000629 hasConcept C181199279 @default.
- W4253000629 hasConcept C185592680 @default.
- W4253000629 hasConcept C193363816 @default.
- W4253000629 hasConcept C2779423947 @default.
- W4253000629 hasConcept C2780925461 @default.
- W4253000629 hasConcept C43617362 @default.
- W4253000629 hasConcept C46749446 @default.
- W4253000629 hasConcept C54689828 @default.
- W4253000629 hasConcept C55493867 @default.
- W4253000629 hasConcept C97428945 @default.
- W4253000629 hasConceptScore W4253000629C101660505 @default.
- W4253000629 hasConceptScore W4253000629C1388644 @default.
- W4253000629 hasConceptScore W4253000629C163994747 @default.
- W4253000629 hasConceptScore W4253000629C181199279 @default.
- W4253000629 hasConceptScore W4253000629C185592680 @default.
- W4253000629 hasConceptScore W4253000629C193363816 @default.
- W4253000629 hasConceptScore W4253000629C2779423947 @default.
- W4253000629 hasConceptScore W4253000629C2780925461 @default.
- W4253000629 hasConceptScore W4253000629C43617362 @default.
- W4253000629 hasConceptScore W4253000629C46749446 @default.
- W4253000629 hasConceptScore W4253000629C54689828 @default.
- W4253000629 hasConceptScore W4253000629C55493867 @default.
- W4253000629 hasConceptScore W4253000629C97428945 @default.
- W4253000629 hasIssue "12" @default.
- W4253000629 hasLocation W42530006291 @default.
- W4253000629 hasOpenAccess W4253000629 @default.
- W4253000629 hasPrimaryLocation W42530006291 @default.
- W4253000629 hasRelatedWork W1594380341 @default.
- W4253000629 hasRelatedWork W1775231007 @default.
- W4253000629 hasRelatedWork W1974350094 @default.
- W4253000629 hasRelatedWork W1999143442 @default.
- W4253000629 hasRelatedWork W2012272263 @default.
- W4253000629 hasRelatedWork W2067120880 @default.
- W4253000629 hasRelatedWork W2395108296 @default.
- W4253000629 hasRelatedWork W257980201 @default.
- W4253000629 hasRelatedWork W2994340503 @default.
- W4253000629 hasRelatedWork W4253000629 @default.
- W4253000629 hasVolume "37" @default.
- W4253000629 isParatext "false" @default.
- W4253000629 isRetracted "false" @default.
- W4253000629 workType "article" @default.