Matches in SemOpenAlex for { <https://semopenalex.org/work/W4253105640> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W4253105640 endingPage "93" @default.
- W4253105640 startingPage "89" @default.
- W4253105640 abstract "The resistance mechanism of Escherichia coli BM2506 to macrolides was found to be due to inactivation. Inactivated oleandomycin was identified as oleandomycin 2′-phosphate by thin-layer chromatography. A new type of macrolide-phosphorylating enzyme, macrolide 2′-phosphotransferase type II (MPH(2′)II), was detected, purified 95-fold and its enzymological properties investigated. MPH(2′)II was a constitutive intracellular enzyme which showed high levels of activity with both 14-member-ring and 16-member-ring macrolides. The optimum pH for the inactivation of oleandomycin was 8.2 and the optimum temperature of the reaction was 40°C. Enzyme activity was lost by heat treatment at 60°C for 1 min. The isoelectric point and Mr of the enzyme were 5.3 and 48 000, respectively. Purine nucleotides, such as ITP, GTP and ATP, were effective as cofactors in the inactivation of macrolides. An inhibitory effect of iodine, EDTA, or divalent cations on MPH(2′)II activity was observed." @default.
- W4253105640 created "2022-05-12" @default.
- W4253105640 creator A5019094466 @default.
- W4253105640 creator A5048566624 @default.
- W4253105640 creator A5081269115 @default.
- W4253105640 date "1992-10-01" @default.
- W4253105640 modified "2023-10-14" @default.
- W4253105640 title "Purification and characterization of macrolide 2â²-phosphotransferase type II from a strain ofEscherichia colihighly resistant to macrolide antibiotics" @default.
- W4253105640 cites W1720602333 @default.
- W4253105640 cites W1761507314 @default.
- W4253105640 cites W1775749144 @default.
- W4253105640 cites W1932091963 @default.
- W4253105640 cites W1942552578 @default.
- W4253105640 cites W1979492674 @default.
- W4253105640 cites W1979612502 @default.
- W4253105640 cites W1990217639 @default.
- W4253105640 cites W1990403697 @default.
- W4253105640 cites W2002259878 @default.
- W4253105640 cites W2005791049 @default.
- W4253105640 cites W2012354564 @default.
- W4253105640 cites W2050629811 @default.
- W4253105640 cites W2054142295 @default.
- W4253105640 cites W2063919745 @default.
- W4253105640 cites W2072978336 @default.
- W4253105640 cites W2078988208 @default.
- W4253105640 cites W2080104471 @default.
- W4253105640 cites W2083929899 @default.
- W4253105640 cites W2090317768 @default.
- W4253105640 cites W2091827585 @default.
- W4253105640 cites W2100977038 @default.
- W4253105640 cites W2133314223 @default.
- W4253105640 cites W2169073081 @default.
- W4253105640 cites W2335059016 @default.
- W4253105640 doi "https://doi.org/10.1111/j.1574-6968.1992.tb05445.x" @default.
- W4253105640 hasPublicationYear "1992" @default.
- W4253105640 type Work @default.
- W4253105640 citedByCount "0" @default.
- W4253105640 crossrefType "journal-article" @default.
- W4253105640 hasAuthorship W4253105640A5019094466 @default.
- W4253105640 hasAuthorship W4253105640A5048566624 @default.
- W4253105640 hasAuthorship W4253105640A5081269115 @default.
- W4253105640 hasBestOaLocation W42531056401 @default.
- W4253105640 hasConcept C178790620 @default.
- W4253105640 hasConcept C181199279 @default.
- W4253105640 hasConcept C185592680 @default.
- W4253105640 hasConcept C2777504003 @default.
- W4253105640 hasConcept C2778018563 @default.
- W4253105640 hasConcept C2778155338 @default.
- W4253105640 hasConcept C2780206985 @default.
- W4253105640 hasConcept C28212737 @default.
- W4253105640 hasConcept C501593827 @default.
- W4253105640 hasConcept C55493867 @default.
- W4253105640 hasConcept C71240020 @default.
- W4253105640 hasConcept C74884574 @default.
- W4253105640 hasConcept C96294017 @default.
- W4253105640 hasConceptScore W4253105640C178790620 @default.
- W4253105640 hasConceptScore W4253105640C181199279 @default.
- W4253105640 hasConceptScore W4253105640C185592680 @default.
- W4253105640 hasConceptScore W4253105640C2777504003 @default.
- W4253105640 hasConceptScore W4253105640C2778018563 @default.
- W4253105640 hasConceptScore W4253105640C2778155338 @default.
- W4253105640 hasConceptScore W4253105640C2780206985 @default.
- W4253105640 hasConceptScore W4253105640C28212737 @default.
- W4253105640 hasConceptScore W4253105640C501593827 @default.
- W4253105640 hasConceptScore W4253105640C55493867 @default.
- W4253105640 hasConceptScore W4253105640C71240020 @default.
- W4253105640 hasConceptScore W4253105640C74884574 @default.
- W4253105640 hasConceptScore W4253105640C96294017 @default.
- W4253105640 hasIssue "1-2" @default.
- W4253105640 hasLocation W42531056401 @default.
- W4253105640 hasOpenAccess W4253105640 @default.
- W4253105640 hasPrimaryLocation W42531056401 @default.
- W4253105640 hasRelatedWork W1969036202 @default.
- W4253105640 hasRelatedWork W2076735414 @default.
- W4253105640 hasRelatedWork W2080104471 @default.
- W4253105640 hasRelatedWork W2092250290 @default.
- W4253105640 hasRelatedWork W2129032931 @default.
- W4253105640 hasRelatedWork W2341058892 @default.
- W4253105640 hasRelatedWork W2409683720 @default.
- W4253105640 hasRelatedWork W2483506585 @default.
- W4253105640 hasRelatedWork W2890144773 @default.
- W4253105640 hasRelatedWork W3006611852 @default.
- W4253105640 hasVolume "97" @default.
- W4253105640 isParatext "false" @default.
- W4253105640 isRetracted "false" @default.
- W4253105640 workType "article" @default.