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- W4253194448 abstract "The hydrolysis of a 5′ cap analogue, diadenosinyl-5′,5′-triphosphate (ApppA), and two dinucleoside monophosphates: adenylyl(3′,5′)adenosine (ApA) and uridylyl(3′,5′)uridine (UpU) promoted by an imidazolate-bridged heterobinuclear copper(II)–zinc(II) complex, Cu(II)-diethylenetriamino-μ-imidazolato–Zn(II)- tris(aminoethyl)amine trisperchlorate (denoted as Cu,Zn-complex in the followings) has been investigated. Kinetic measurements were performed in order to explore the effects of pH, the total concentration of the Cu,Zn-complex and temperature on the cleavage rate. The catalytic activity of the Cu,Zn-complex was quantified by pseudo-first-order rate constants obtained in the excess of the cleaving agent. The results show that the Cu,Zn-complex and its deprotonated forms have phosphoesterase activity and with ApppA the metal complex promoted cleavage takes place selectively within the triphosphate bridge." @default.
- W4253194448 created "2022-05-12" @default.
- W4253194448 date "1973-01-01" @default.
- W4253194448 modified "2023-09-25" @default.
- W4253194448 title "2616. Uses for diethyl pyrocarbonate in biochemistry" @default.
- W4253194448 doi "https://doi.org/10.1016/s0015-6264(73)80451-1" @default.
- W4253194448 hasPublicationYear "1973" @default.
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