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- W4253275973 abstract "This chapter describes the isolation and culture of neonatal mouse calvarial osteoblasts. This primary cell population is obtained by sequential enzymatic digestion of the calvarial bone matrix and is capable of differentiating in vitro into mature osteoblasts that deposit a collagen extracellular matrix and form mineralized bone nodules. Maturation of the cultures can be monitored by gene expression analyses and staining for the presence of alkaline phosphatase or matrix mineralization. This culture system, therefore, provides a powerful model in which to test how various experimental conditions, such as the manipulation of gene expression, may affect osteoblast maturation and/or function." @default.
- W4253275973 created "2022-05-12" @default.
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- W4253275973 date "2020-11-17" @default.
- W4253275973 modified "2023-10-17" @default.
- W4253275973 title "Isolation and Culture of Neonatal Mouse Calvarial Osteoblasts" @default.
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- W4253275973 doi "https://doi.org/10.1007/978-1-0716-1028-2_26" @default.
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