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• W4253422011 abstract "Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a strategy that simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, using homologous recombination in bacteria rather than in eukaryotic cells. After transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes." @default.
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• W4253422011 date "1998-03-03" @default.
• W4253422011 modified "2023-10-16" @default.
• W4253422011 title "A simplified system for generating recombinant adenoviruses" @default.
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• W4253422011 doi "https://doi.org/10.1073/pnas.95.5.2509" @default.
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