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- W4253485358 abstract "Abstract Data were collected from 30 strains of Vibrio and sampled on different organs (brain, hemorrhagic site and digestive tract) of Sciaenops ocellatus infection. The results showed that the nucleotide sequences 16S rRNA region are highly similar to those of V. alginolyticus , V. azureus , V. fluvialis, V. natriengens and V. orientalis, which were published on Genbank and other, ranging from 98.05 to 100 %. The digestive tract has the most common Vibrio strains ( V. alginolyticus [ 16] V. azureu s [7] and V. fluvialis ). Thereout, 25 of 30 strains of Vibrio contained from 1 to 3 toxin genes, except for V. parahaemolyticus . Six parameters were used to measure the DNA polymorphism of 33 homologous DNA sequences in this Vibrio bacteria population. The results indicated that the number of separate polymorphic sites (S), the total number of mutant sites (Eta), the number of haplotype (h), the haplotype diversity (Hd), the average number of nucleotide differences (k), the nucleotide diversity (Pi) were 98 (S), 103 (Eta), 9 (h), 0.887 ± 0.032 (Hd), 25.789 (k) and 17.980x10-3 ± 0.003 (Pi), respectively (P < 0,05). The G+C contentover 1434 sites positions of nucleotide sequences accounted for 0.542. The phylogenetic tree showed that these strains are divided into six groups. As observed, the appearance of isolated Vibrio on 3 organs of fish ( S. ocellatus ) which are hemorrhagic include V. azureus (27,67 %), V. alginolyticus (50 %), V. orientalis (6,67 %) and V. fluvialis (16,67 %). Through this result, it is found that the diversity of Vibrio species that appeared on the red drum was used in the 16S rRNA region and the presence of toxin genes in these Vibrio species." @default.
- W4253485358 created "2022-05-12" @default.
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- W4253485358 date "2020-10-29" @default.
- W4253485358 modified "2023-09-30" @default.
- W4253485358 title "The identification and determination of toxin genes of Vibrio strains causing hemorrhagic disease on red drum (Sciaenops ocellatus) using PCR method" @default.
- W4253485358 doi "https://doi.org/10.21203/rs.3.rs-64857/v4" @default.
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