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- W4253485687 abstract "The application of Phaffia rhodozyma as an astaxanthin source in the aquaculture industry is limited because of the low carotenoid content of natural isolates. We constructed carotenoid hyperproducing hybrids of P. rhodozyma by protoplast fusion from parental strains that produced approx. 1600 μg carotenoid/g yeast and were auxotrophic for tryptophan, leucine, methionine or arginine. The hybrids were stable and consistently produced > 2000 μg carotenoid/g yeast. Karyogamy was confirmed by the isolation of recombinants after mitotic segregation of parental auxotrophic genetic markers, the increased content of DNA/cell and the presence of a single nucleus per cell. The results of this study indicated that protoplast fusion is promising for strain improvement in P. rhodozyma." @default.
- W4253485687 created "2022-05-12" @default.
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- W4253485687 date "1992-06-15" @default.
- W4253485687 modified "2023-10-17" @default.
- W4253485687 title "Strain improvement of Phaffia rhodozyma by protoplast fusion" @default.
- W4253485687 doi "https://doi.org/10.1016/0378-1097(92)90466-2" @default.
- W4253485687 hasPublicationYear "1992" @default.
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