Matches in SemOpenAlex for { <https://semopenalex.org/work/W4253676743> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4253676743 endingPage "1085" @default.
- W4253676743 startingPage "1079" @default.
- W4253676743 abstract "The effect of catalytic subunit (C) of cyclic AMP-dependent protein kinase on cell growth kinetics of BHK cells was assessed by microinjection with chicken erythrocyte ghosts as vehicles for introduction of the protein into the cytosol of large populations of cells. The advantage in using chicken erythrocytes for microinjection is that the inactive erythrocyte nuclei serve as a probe for identifying and analyzing microinjection events. By utilizing this procedure, BHK cells were microinjected with an amount of C that was 5- to 10-fold greater than their endogenous levels. Growth kinetics were analyzed by [3H]thymidine incorporation and autoradiography. Cells were stained after autoradiography to more clearly reveal the chicken nuclei, and at each time point, cells were categorized into four groups: (i) not microinjected, not in S phase, (ii) not microinjected, in S phase, (iii) microinjected, not in S phase, (iv) microinjected, in S phase. Those cells not microinjected served as internal controls. Two experimental protocols were used to test the notion that C is involved in blocking cell progression through G1 phase of the cell cycle. First, cells were arrested in G0 phase by serum deprivation, microinjected with C or control proteins, and stimulated to proceed to S phase by the addition of serum or purified growth factors. Second, cells were collected in mitosis, microinjected with C or control proteins, and stimulated to proceed to S phase by the addition of serum. The results of these studies indicate that a 5- to 10-fold increase in the intracellular concentration of C is not a sufficient signal to arrest cell growth in G1 phase. Thus, growth-inhibitory effects of cyclic AMP on BHK cells are unlikely to be the result of activation of cyclic AMP-dependent protein kinase." @default.
- W4253676743 created "2022-05-12" @default.
- W4253676743 creator A5028683429 @default.
- W4253676743 creator A5074060115 @default.
- W4253676743 date "1984-06-01" @default.
- W4253676743 modified "2023-10-12" @default.
- W4253676743 title "Analysis of BHK cell growth kinetics after microinjection of catalytic subunit of cyclic AMP-dependent protein kinase." @default.
- W4253676743 doi "https://doi.org/10.1128/mcb.4.6.1079" @default.
- W4253676743 hasPublicationYear "1984" @default.
- W4253676743 type Work @default.
- W4253676743 citedByCount "2" @default.
- W4253676743 crossrefType "journal-article" @default.
- W4253676743 hasAuthorship W4253676743A5028683429 @default.
- W4253676743 hasAuthorship W4253676743A5074060115 @default.
- W4253676743 hasBestOaLocation W42536767431 @default.
- W4253676743 hasConcept C104292427 @default.
- W4253676743 hasConcept C104317684 @default.
- W4253676743 hasConcept C1491633281 @default.
- W4253676743 hasConcept C153911025 @default.
- W4253676743 hasConcept C181199279 @default.
- W4253676743 hasConcept C184235292 @default.
- W4253676743 hasConcept C29537977 @default.
- W4253676743 hasConcept C33263850 @default.
- W4253676743 hasConcept C54355233 @default.
- W4253676743 hasConcept C55493867 @default.
- W4253676743 hasConcept C62112901 @default.
- W4253676743 hasConcept C62525196 @default.
- W4253676743 hasConcept C79879829 @default.
- W4253676743 hasConcept C81885089 @default.
- W4253676743 hasConcept C85813293 @default.
- W4253676743 hasConcept C86803240 @default.
- W4253676743 hasConcept C93304396 @default.
- W4253676743 hasConcept C95444343 @default.
- W4253676743 hasConcept C97029542 @default.
- W4253676743 hasConcept C98539663 @default.
- W4253676743 hasConceptScore W4253676743C104292427 @default.
- W4253676743 hasConceptScore W4253676743C104317684 @default.
- W4253676743 hasConceptScore W4253676743C1491633281 @default.
- W4253676743 hasConceptScore W4253676743C153911025 @default.
- W4253676743 hasConceptScore W4253676743C181199279 @default.
- W4253676743 hasConceptScore W4253676743C184235292 @default.
- W4253676743 hasConceptScore W4253676743C29537977 @default.
- W4253676743 hasConceptScore W4253676743C33263850 @default.
- W4253676743 hasConceptScore W4253676743C54355233 @default.
- W4253676743 hasConceptScore W4253676743C55493867 @default.
- W4253676743 hasConceptScore W4253676743C62112901 @default.
- W4253676743 hasConceptScore W4253676743C62525196 @default.
- W4253676743 hasConceptScore W4253676743C79879829 @default.
- W4253676743 hasConceptScore W4253676743C81885089 @default.
- W4253676743 hasConceptScore W4253676743C85813293 @default.
- W4253676743 hasConceptScore W4253676743C86803240 @default.
- W4253676743 hasConceptScore W4253676743C93304396 @default.
- W4253676743 hasConceptScore W4253676743C95444343 @default.
- W4253676743 hasConceptScore W4253676743C97029542 @default.
- W4253676743 hasConceptScore W4253676743C98539663 @default.
- W4253676743 hasIssue "6" @default.
- W4253676743 hasLocation W42536767431 @default.
- W4253676743 hasLocation W42536767432 @default.
- W4253676743 hasLocation W42536767433 @default.
- W4253676743 hasOpenAccess W4253676743 @default.
- W4253676743 hasPrimaryLocation W42536767431 @default.
- W4253676743 hasRelatedWork W1999714242 @default.
- W4253676743 hasRelatedWork W2054608659 @default.
- W4253676743 hasRelatedWork W2059386627 @default.
- W4253676743 hasRelatedWork W2066519212 @default.
- W4253676743 hasRelatedWork W2072481810 @default.
- W4253676743 hasRelatedWork W2146508653 @default.
- W4253676743 hasRelatedWork W2278670241 @default.
- W4253676743 hasRelatedWork W2363880822 @default.
- W4253676743 hasRelatedWork W4244660752 @default.
- W4253676743 hasRelatedWork W4280493527 @default.
- W4253676743 hasVolume "4" @default.
- W4253676743 isParatext "false" @default.
- W4253676743 isRetracted "false" @default.
- W4253676743 workType "article" @default.