FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4253720221> ?p ?o ?g. }

• W4253720221 endingPage "3754" @default.
• W4253720221 startingPage "3745" @default.
• W4253720221 abstract "The U-A10 cell line, a doxorubicin-selected variant of human U-937 myeloid leukemia cells, exhibits a redistribution of anthracyclines into a expanded vesicular compartment. The acidic nature of this compartment was confirmed by vital staining with a pH sensitive dye, LysoSensor yellow/blue DND-160. Identification of the vesicular compartment was performed by immunofluorescence analysis. Staining for the LAMP-1 and LAMP-2 antigens showed that the vesicles are enlarged lysosomes that are eccentrically placed near the nucleus of U-A10 cells. By contrast, the expression of the multidrug resistance-associated protein and the P-glycoprotein was observed predominately on the plasma membrane of the drug-resistant cells. The accumulation of daunorubicin into cellular compartments was quantified using radiolabeled drug. Exposing cells to 3[H]-daunorubicin and then isolating intact nuclei showed that nuclei from U-A10 cells accumulated twofold to threefold less anthracycline than nuclei from U-937 cells. However, when nuclei were isolated first and then exposed to 3[H]-daunorubicin, little difference in net nuclear drug accumulation was detected. Cytoplasts prepared from U-A10 and U-937 cells were exposed to 3[H]-daunorubicin to measure cytoplasmic drug accumulation. At external daunorubicin concentrations of 100 ng/mL or higher, cytoplasts from U-A10 cells accumulated significantly more daunorubicin than cytoplasts from U-937 cells. Moreover, studies with the lysosomotropic agent chloroquine showed that U-A10 cells accumulated twofold more chloroquine and showed twofold enhanced sensitivity to this agent as compared with parental U-937 cells. Fluorescence microscopy showed that chloroquine affects vesicular anthracycline sequestration in U-A10 cells with an associated increase in daunorubicin nuclear fluorescence. Although chloroquine did not alter anthracycline cytotoxicity in parental cells, it restored daunorubicin and doxorubicin sensitivity to U-A10 cells. Taken together, these studies demonstrate that U-A10 cells exhibit a redistribution of the lysosomal compartment. The trapping of drug into an expanded acidic vesicular compartment results in decreased nuclear drug accumulation and decreased cytotoxicity. Lysosomotropic agents, such as chloroquine, warrant further study as modulators of this acquired drug-resistance phenotype." @default.
• W4253720221 created "2022-05-12" @default.
• W4253720221 creator A5020969371 @default.
• W4253720221 creator A5040869308 @default.
• W4253720221 creator A5070095260 @default.
• W4253720221 creator A5086832276 @default.
• W4253720221 date "1997-05-15" @default.
• W4253720221 modified "2023-10-16" @default.
• W4253720221 title "Vesicular Anthracycline Accumulation in Doxorubicin-Selected U-937 Cells: Participation of Lysosomes" @default.
• W4253720221 cites W1582548909 @default.
• W4253720221 cites W1594196316 @default.
• W4253720221 cites W1645373081 @default.
• W4253720221 cites W1956702905 @default.
• W4253720221 cites W1978111569 @default.
• W4253720221 cites W1999795482 @default.
• W4253720221 cites W2003263277 @default.
• W4253720221 cites W2007931369 @default.
• W4253720221 cites W2008840383 @default.
• W4253720221 cites W2016254453 @default.
• W4253720221 cites W2017319661 @default.
• W4253720221 cites W2017731672 @default.
• W4253720221 cites W2018652769 @default.
• W4253720221 cites W2019947202 @default.
• W4253720221 cites W2026606228 @default.
• W4253720221 cites W2056600059 @default.
• W4253720221 cites W2058273909 @default.
• W4253720221 cites W2061660948 @default.
• W4253720221 cites W2063795262 @default.
• W4253720221 cites W2067898376 @default.
• W4253720221 cites W2077790247 @default.
• W4253720221 cites W2080826402 @default.
• W4253720221 cites W2114918609 @default.
• W4253720221 cites W2130456190 @default.
• W4253720221 cites W2151423449 @default.
• W4253720221 cites W2168070336 @default.
• W4253720221 cites W2171344824 @default.
• W4253720221 cites W2234746365 @default.
• W4253720221 cites W4211222589 @default.
• W4253720221 cites W4229988036 @default.
• W4253720221 cites W4256031604 @default.
• W4253720221 doi "https://doi.org/10.1182/blood.v89.10.3745.3745_3745_3754" @default.
• W4253720221 hasPublicationYear "1997" @default.
• W4253720221 type Work @default.
• W4253720221 citedByCount "3" @default.
• W4253720221 countsByYear W42537202212020 @default.
• W4253720221 countsByYear W42537202212021 @default.
• W4253720221 countsByYear W42537202212023 @default.
• W4253720221 crossrefType "journal-article" @default.
• W4253720221 hasAuthorship W4253720221A5020969371 @default.
• W4253720221 hasAuthorship W4253720221A5040869308 @default.
• W4253720221 hasAuthorship W4253720221A5070095260 @default.
• W4253720221 hasAuthorship W4253720221A5086832276 @default.
• W4253720221 hasBestOaLocation W42537202211 @default.
• W4253720221 hasConcept C121608353 @default.
• W4253720221 hasConcept C153911025 @default.
• W4253720221 hasConcept C190062978 @default.
• W4253720221 hasConcept C190546358 @default.
• W4253720221 hasConcept C203014093 @default.
• W4253720221 hasConcept C2776802502 @default.
• W4253720221 hasConcept C2778461978 @default.
• W4253720221 hasConcept C2781021840 @default.
• W4253720221 hasConcept C530470458 @default.
• W4253720221 hasConcept C54355233 @default.
• W4253720221 hasConcept C81885089 @default.
• W4253720221 hasConcept C86803240 @default.
• W4253720221 hasConcept C95444343 @default.
• W4253720221 hasConceptScore W4253720221C121608353 @default.
• W4253720221 hasConceptScore W4253720221C153911025 @default.
• W4253720221 hasConceptScore W4253720221C190062978 @default.
• W4253720221 hasConceptScore W4253720221C190546358 @default.
• W4253720221 hasConceptScore W4253720221C203014093 @default.
• W4253720221 hasConceptScore W4253720221C2776802502 @default.
• W4253720221 hasConceptScore W4253720221C2778461978 @default.
• W4253720221 hasConceptScore W4253720221C2781021840 @default.
• W4253720221 hasConceptScore W4253720221C530470458 @default.
• W4253720221 hasConceptScore W4253720221C54355233 @default.
• W4253720221 hasConceptScore W4253720221C81885089 @default.
• W4253720221 hasConceptScore W4253720221C86803240 @default.
• W4253720221 hasConceptScore W4253720221C95444343 @default.
• W4253720221 hasIssue "10" @default.
• W4253720221 hasLocation W42537202211 @default.
• W4253720221 hasOpenAccess W4253720221 @default.
• W4253720221 hasPrimaryLocation W42537202211 @default.
• W4253720221 hasRelatedWork W12212138 @default.
• W4253720221 hasRelatedWork W16782557 @default.
• W4253720221 hasRelatedWork W22944490 @default.
• W4253720221 hasRelatedWork W25000151 @default.
• W4253720221 hasRelatedWork W25992215 @default.
• W4253720221 hasRelatedWork W32238353 @default.
• W4253720221 hasRelatedWork W36235900 @default.
• W4253720221 hasRelatedWork W4017593 @default.
• W4253720221 hasRelatedWork W40197506 @default.
• W4253720221 hasRelatedWork W47482700 @default.
• W4253720221 hasVolume "89" @default.
• W4253720221 isParatext "false" @default.
• W4253720221 isRetracted "false" @default.
• W4253720221 workType "article" @default.