Matches in SemOpenAlex for { <https://semopenalex.org/work/W4253732851> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W4253732851 abstract "Abstract Objective: The aim of this study was to compare the quality and efficiency of exosomes extracted from knee joint effusion by different methods, laying a foundation for further research on exosomes from knee joint effusion. Methods: To separate and extract joint exosomes by 8% polyethylene glycol (PEG) precipitation method, ultracentrifugation method (UC) and ultrafiltration with exclusion chromatography (SECF). Transmission electron microscopy (TEM) and nanoparticle tracing technology (NTA) were used to detect particle morphology and particle size, and Western Blot (WB) was used to detect marker proteins of granule surface (CD9, CD63, Flotillin-1 and calnexin). Results: Three methods separated vesicle-like round particles from joint effusion successfully. The results of TEM show that the particles obtained by the three extraction methods are round or oval vesicles, the plasma membrane is intact, the size is different, and the diameter is distributed between 30 and 150 nm. Compared with SECF group, PEG group had more background particle impurities. and the broken vesicle fragments can be seen in the UC group; The results of NTA show that the main peaks of the three groups of particles are between 100-120nm, and the particle concentration is greater than 1×10 10 /ml; The results of WB show that the protein expressions of CD9, CD63 and Flotillin-1 in the suspension extracted by the three methods were higher, the expression of calnexin protein in the PEG group was higher than the UC group and SECF group. Conclusion: The three extraction methods can extract the exosomes of joint effusion successfully. The quality of exosomes obtained by the SECF method is relatively high, while the PEG precipitation method contains a small amount of impurity particles. UC method does not guarantee the integrity of exosomes. In summary, we recommend the SECF method to extract and isolate joint effusion exudates-derived exosomes when further studying the role of exosomes in the pathogenesis of knee osteoarthritis." @default.
- W4253732851 created "2022-05-12" @default.
- W4253732851 creator A5004425277 @default.
- W4253732851 creator A5019576898 @default.
- W4253732851 creator A5036281846 @default.
- W4253732851 creator A5051120464 @default.
- W4253732851 creator A5060551332 @default.
- W4253732851 creator A5075234529 @default.
- W4253732851 creator A5082540938 @default.
- W4253732851 date "2020-10-23" @default.
- W4253732851 modified "2023-09-28" @default.
- W4253732851 title "Extraction and quality evaluation of exosomes from joint effusion" @default.
- W4253732851 doi "https://doi.org/10.21203/rs.3.rs-49025/v2" @default.
- W4253732851 hasPublicationYear "2020" @default.
- W4253732851 type Work @default.
- W4253732851 citedByCount "0" @default.
- W4253732851 crossrefType "posted-content" @default.
- W4253732851 hasAuthorship W4253732851A5004425277 @default.
- W4253732851 hasAuthorship W4253732851A5019576898 @default.
- W4253732851 hasAuthorship W4253732851A5036281846 @default.
- W4253732851 hasAuthorship W4253732851A5051120464 @default.
- W4253732851 hasAuthorship W4253732851A5060551332 @default.
- W4253732851 hasAuthorship W4253732851A5075234529 @default.
- W4253732851 hasAuthorship W4253732851A5082540938 @default.
- W4253732851 hasBestOaLocation W42537328511 @default.
- W4253732851 hasConcept C10138342 @default.
- W4253732851 hasConcept C104317684 @default.
- W4253732851 hasConcept C130316041 @default.
- W4253732851 hasConcept C145059251 @default.
- W4253732851 hasConcept C153911025 @default.
- W4253732851 hasConcept C162324750 @default.
- W4253732851 hasConcept C185592680 @default.
- W4253732851 hasConcept C192562407 @default.
- W4253732851 hasConcept C20518536 @default.
- W4253732851 hasConcept C2776385711 @default.
- W4253732851 hasConcept C2776415932 @default.
- W4253732851 hasConcept C2776964284 @default.
- W4253732851 hasConcept C2778120352 @default.
- W4253732851 hasConcept C41625074 @default.
- W4253732851 hasConcept C43617362 @default.
- W4253732851 hasConcept C4725764 @default.
- W4253732851 hasConcept C54400483 @default.
- W4253732851 hasConcept C55493867 @default.
- W4253732851 hasConcept C86803240 @default.
- W4253732851 hasConceptScore W4253732851C10138342 @default.
- W4253732851 hasConceptScore W4253732851C104317684 @default.
- W4253732851 hasConceptScore W4253732851C130316041 @default.
- W4253732851 hasConceptScore W4253732851C145059251 @default.
- W4253732851 hasConceptScore W4253732851C153911025 @default.
- W4253732851 hasConceptScore W4253732851C162324750 @default.
- W4253732851 hasConceptScore W4253732851C185592680 @default.
- W4253732851 hasConceptScore W4253732851C192562407 @default.
- W4253732851 hasConceptScore W4253732851C20518536 @default.
- W4253732851 hasConceptScore W4253732851C2776385711 @default.
- W4253732851 hasConceptScore W4253732851C2776415932 @default.
- W4253732851 hasConceptScore W4253732851C2776964284 @default.
- W4253732851 hasConceptScore W4253732851C2778120352 @default.
- W4253732851 hasConceptScore W4253732851C41625074 @default.
- W4253732851 hasConceptScore W4253732851C43617362 @default.
- W4253732851 hasConceptScore W4253732851C4725764 @default.
- W4253732851 hasConceptScore W4253732851C54400483 @default.
- W4253732851 hasConceptScore W4253732851C55493867 @default.
- W4253732851 hasConceptScore W4253732851C86803240 @default.
- W4253732851 hasLocation W42537328511 @default.
- W4253732851 hasLocation W42537328512 @default.
- W4253732851 hasLocation W42537328513 @default.
- W4253732851 hasOpenAccess W4253732851 @default.
- W4253732851 hasPrimaryLocation W42537328511 @default.
- W4253732851 hasRelatedWork W2338060077 @default.
- W4253732851 hasRelatedWork W2338671470 @default.
- W4253732851 hasRelatedWork W2440935561 @default.
- W4253732851 hasRelatedWork W2576826502 @default.
- W4253732851 hasRelatedWork W2889577239 @default.
- W4253732851 hasRelatedWork W2906374810 @default.
- W4253732851 hasRelatedWork W3003542953 @default.
- W4253732851 hasRelatedWork W3158610674 @default.
- W4253732851 hasRelatedWork W4232923066 @default.
- W4253732851 hasRelatedWork W4313365844 @default.
- W4253732851 isParatext "false" @default.
- W4253732851 isRetracted "false" @default.
- W4253732851 workType "article" @default.