Matches in SemOpenAlex for { <https://semopenalex.org/work/W4254199963> ?p ?o ?g. }
Showing items 1 to 59 of
59
with 100 items per page.
- W4254199963 abstract "Abstract Cells in taste buds are closely packed, with little extracellular space. Tight junctions and other barriers further limit permeability and may result in buildup of extracellular K + following action potentials. In many tissues, inwardly rectifying K channels such as the renal outer medullary K (ROMK) channel (also called Kir1.1 and derived from the Kcnj1 gene) help to redistribute K + . Using reverse‐transcription polymerase chain reaction (RT‐PCR), we defined ROMK splice variants in mouse kidney and report here the expression of a single one of these, ROMK2, in a subset of mouse taste cells. With quantitative (q)RT‐PCR, we show the abundance of ROMK mRNA in taste buds is vallate > foliate ≫ palate ≫ fungiform. ROMK protein follows the same pattern of prevalence as mRNA, and is essentially undetectable by immunohistochemistry in fungiform taste buds. ROMK protein is localized to the apical tips of a subset of taste cells. Using tissues from PLCβ2‐GFP and GAD‐GFP transgenic mice, we show that ROMK is not found in PLCβ2‐expressing type II/receptor cells or in GAD‐expressing type III/presynaptic cells. Instead, ROMK is found, by single‐cell RT‐PCR and immunofluorescence, in most cells that are positive for the taste glial cell marker, Ectonucleotidase2. ROMK is precisely localized to the apical tips of these cells, at and above apical tight junctions. We propose that in taste buds, ROMK in type I/glial‐like cells may serve a homeostatic function, excreting excess K + through the apical pore, and allowing excitable taste cells to maintain a hyperpolarized resting membrane potential. J. Comp. Neurol. 517:1–14, 2009. © 2009 Wiley‐Liss, Inc." @default.
- W4254199963 created "2022-05-12" @default.
- W4254199963 creator A5021571464 @default.
- W4254199963 creator A5037354482 @default.
- W4254199963 creator A5060767884 @default.
- W4254199963 creator A5075524197 @default.
- W4254199963 creator A5083455588 @default.
- W4254199963 date "2009-08-26" @default.
- W4254199963 modified "2023-10-12" @default.
- W4254199963 title "Inward rectifier channel, ROMK, is localized to the apical tips of glial-like cells in mouse taste buds" @default.
- W4254199963 doi "https://doi.org/10.1002/cne.22196" @default.
- W4254199963 hasPublicationYear "2009" @default.
- W4254199963 type Work @default.
- W4254199963 citedByCount "0" @default.
- W4254199963 crossrefType "journal-article" @default.
- W4254199963 hasAuthorship W4254199963A5021571464 @default.
- W4254199963 hasAuthorship W4254199963A5037354482 @default.
- W4254199963 hasAuthorship W4254199963A5060767884 @default.
- W4254199963 hasAuthorship W4254199963A5075524197 @default.
- W4254199963 hasAuthorship W4254199963A5083455588 @default.
- W4254199963 hasBestOaLocation W42541999631 @default.
- W4254199963 hasConcept C126322002 @default.
- W4254199963 hasConcept C153911025 @default.
- W4254199963 hasConcept C185592680 @default.
- W4254199963 hasConcept C28406088 @default.
- W4254199963 hasConcept C3018436504 @default.
- W4254199963 hasConcept C41625074 @default.
- W4254199963 hasConcept C55493867 @default.
- W4254199963 hasConcept C71924100 @default.
- W4254199963 hasConcept C86803240 @default.
- W4254199963 hasConcept C95444343 @default.
- W4254199963 hasConceptScore W4254199963C126322002 @default.
- W4254199963 hasConceptScore W4254199963C153911025 @default.
- W4254199963 hasConceptScore W4254199963C185592680 @default.
- W4254199963 hasConceptScore W4254199963C28406088 @default.
- W4254199963 hasConceptScore W4254199963C3018436504 @default.
- W4254199963 hasConceptScore W4254199963C41625074 @default.
- W4254199963 hasConceptScore W4254199963C55493867 @default.
- W4254199963 hasConceptScore W4254199963C71924100 @default.
- W4254199963 hasConceptScore W4254199963C86803240 @default.
- W4254199963 hasConceptScore W4254199963C95444343 @default.
- W4254199963 hasIssue "1" @default.
- W4254199963 hasLocation W42541999631 @default.
- W4254199963 hasOpenAccess W4254199963 @default.
- W4254199963 hasPrimaryLocation W42541999631 @default.
- W4254199963 hasRelatedWork W1986936937 @default.
- W4254199963 hasRelatedWork W2002665694 @default.
- W4254199963 hasRelatedWork W2024392898 @default.
- W4254199963 hasRelatedWork W2028679378 @default.
- W4254199963 hasRelatedWork W2030317873 @default.
- W4254199963 hasRelatedWork W2050711297 @default.
- W4254199963 hasRelatedWork W2125434199 @default.
- W4254199963 hasRelatedWork W2952941127 @default.
- W4254199963 hasRelatedWork W750390717 @default.
- W4254199963 hasRelatedWork W190756422 @default.
- W4254199963 hasVolume "517" @default.
- W4254199963 isParatext "false" @default.
- W4254199963 isRetracted "false" @default.
- W4254199963 workType "article" @default.