Matches in SemOpenAlex for { <https://semopenalex.org/work/W4254301708> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W4254301708 endingPage "444" @default.
- W4254301708 startingPage "435" @default.
- W4254301708 abstract "An important mechanism of platelet regulation is the formation of the second messenger diacylglycerol (DAG) and the activation of protein kinase C (PKC). Our previous studies suggested that the DAG/PKC pathway plays an important role in the induction of secretion and secondary aggregation rather than the earlier events of shape change and primary aggregation. We therefore examined the hypothesis that the delayed effects of PKC on platelets may result from delayed accumulation of DAG. The kinetics of DAG formation in human platelets were determined. When platelets were stimulated with gamma-thrombin, the largest phase of DAG accumulation was delayed for 0.6 to 0.8 minutes and DAG mass levels remained elevated for at least 2 minutes. In platelets stimulated with collagen, DAG accumulation was delayed for 1.0 to 1.2 minutes and DAG mass levels remained elevated for at least 3 minutes after stimulation. Sustained DAG production was also associated with sustained activation of PKC as monitored by phosphorylation of the 40- Kd substrate of PKC. The physiologic significance of delayed DAG accumulation was investigated using the cell-permeable DAG analog, dioctanoylglycerol (diC8). In synergy with subthreshold gamma-thrombin or collagen, exogenous diC8 reconstituted platelet activation. The optimal timing of addition of diC8 was 0.5 minutes after stimulation with gamma-thrombin or collagen. These kinetics were similar to those of endogenous DAG accumulation. These studies underscore the importance of a delayed accumulative phase of DAG generation as a mechanism controlling the onset of platelet secretion and irreversible aggregation." @default.
- W4254301708 created "2022-05-12" @default.
- W4254301708 creator A5054118410 @default.
- W4254301708 creator A5073975749 @default.
- W4254301708 date "1991-07-15" @default.
- W4254301708 modified "2023-09-29" @default.
- W4254301708 title "Delayed accumulation of diacylglycerol in platelets as a mechanism for regulation of onset of aggregation and secretion" @default.
- W4254301708 doi "https://doi.org/10.1182/blood.v78.2.435.bloodjournal782435" @default.
- W4254301708 hasPublicationYear "1991" @default.
- W4254301708 type Work @default.
- W4254301708 citedByCount "0" @default.
- W4254301708 crossrefType "journal-article" @default.
- W4254301708 hasAuthorship W4254301708A5054118410 @default.
- W4254301708 hasAuthorship W4254301708A5073975749 @default.
- W4254301708 hasBestOaLocation W42543017081 @default.
- W4254301708 hasConcept C11960822 @default.
- W4254301708 hasConcept C126322002 @default.
- W4254301708 hasConcept C134018914 @default.
- W4254301708 hasConcept C163235415 @default.
- W4254301708 hasConcept C181199279 @default.
- W4254301708 hasConcept C185592680 @default.
- W4254301708 hasConcept C195794163 @default.
- W4254301708 hasConcept C24998067 @default.
- W4254301708 hasConcept C2776387844 @default.
- W4254301708 hasConcept C2777292125 @default.
- W4254301708 hasConcept C3018697912 @default.
- W4254301708 hasConcept C36020004 @default.
- W4254301708 hasConcept C49039625 @default.
- W4254301708 hasConcept C55493867 @default.
- W4254301708 hasConcept C62478195 @default.
- W4254301708 hasConcept C71924100 @default.
- W4254301708 hasConcept C86803240 @default.
- W4254301708 hasConcept C89560881 @default.
- W4254301708 hasConcept C95444343 @default.
- W4254301708 hasConceptScore W4254301708C11960822 @default.
- W4254301708 hasConceptScore W4254301708C126322002 @default.
- W4254301708 hasConceptScore W4254301708C134018914 @default.
- W4254301708 hasConceptScore W4254301708C163235415 @default.
- W4254301708 hasConceptScore W4254301708C181199279 @default.
- W4254301708 hasConceptScore W4254301708C185592680 @default.
- W4254301708 hasConceptScore W4254301708C195794163 @default.
- W4254301708 hasConceptScore W4254301708C24998067 @default.
- W4254301708 hasConceptScore W4254301708C2776387844 @default.
- W4254301708 hasConceptScore W4254301708C2777292125 @default.
- W4254301708 hasConceptScore W4254301708C3018697912 @default.
- W4254301708 hasConceptScore W4254301708C36020004 @default.
- W4254301708 hasConceptScore W4254301708C49039625 @default.
- W4254301708 hasConceptScore W4254301708C55493867 @default.
- W4254301708 hasConceptScore W4254301708C62478195 @default.
- W4254301708 hasConceptScore W4254301708C71924100 @default.
- W4254301708 hasConceptScore W4254301708C86803240 @default.
- W4254301708 hasConceptScore W4254301708C89560881 @default.
- W4254301708 hasConceptScore W4254301708C95444343 @default.
- W4254301708 hasIssue "2" @default.
- W4254301708 hasLocation W42543017081 @default.
- W4254301708 hasOpenAccess W4254301708 @default.
- W4254301708 hasPrimaryLocation W42543017081 @default.
- W4254301708 hasRelatedWork W10341653 @default.
- W4254301708 hasRelatedWork W17727941 @default.
- W4254301708 hasRelatedWork W19367934 @default.
- W4254301708 hasRelatedWork W26771115 @default.
- W4254301708 hasRelatedWork W28533922 @default.
- W4254301708 hasRelatedWork W30942452 @default.
- W4254301708 hasRelatedWork W31917496 @default.
- W4254301708 hasRelatedWork W4738867 @default.
- W4254301708 hasRelatedWork W4880839 @default.
- W4254301708 hasRelatedWork W5184045 @default.
- W4254301708 hasVolume "78" @default.
- W4254301708 isParatext "false" @default.
- W4254301708 isRetracted "false" @default.
- W4254301708 workType "article" @default.