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- W4254315415 abstract "Cholera toxoid is an established tool for use in cellular tracing in neuroscience and cell biology. We use a sortase-labelling approach to generate site-specifically <i>N</i>-terminally modified variants of both the A2-B<sub>5</sub> heterohexamer and B<sub>5</sub> pentamer forms of the toxoid. Both forms of the toxoid are endocytosed by GM1-positive mammalian cells, and while the heterohexameric toxoid was principally localized in the ER, the B<sub>5</sub> pentamer showed an unexpected localization in the <i>medial/trans</i> Golgi. This study suggests a future role for specifically-labelled cholera toxoids in live-cell imaging beyond their current applications in neuronal tracing and labelling of lipid-rafts in fixed cells." @default.
- W4254315415 created "2022-05-12" @default.
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- W4254315415 date "2019-07-29" @default.
- W4254315415 modified "2023-09-23" @default.
- W4254315415 title "Sortase-Modified Cholera Toxoids Show Specific Golgi Localization" @default.
- W4254315415 doi "https://doi.org/10.26434/chemrxiv.9125201" @default.
- W4254315415 hasPublicationYear "2019" @default.
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