Matches in SemOpenAlex for { <https://semopenalex.org/work/W4254702679> ?p ?o ?g. }
Showing items 1 to 58 of
58
with 100 items per page.
- W4254702679 endingPage "215" @default.
- W4254702679 startingPage "215" @default.
- W4254702679 abstract "Oxygenated derivatives of cholesterol have important functions in many biochemical processes. These oxysterols are difficult to study because of their low physiological concentrations, the facile formation of cholesterol autoxidation artifacts, and lack of information on their chromatographic behavior. Focusing on metabolites and autoxidation products of cholesterol, we have documented the chromatographic mobilities of 35 oxysterols under a variety of conditions: eight solvent systems for thin-layer chromatography on silica gel, several mobile phases for reversed-phase high-performance liquid chromatography (HPLC), and two types of stationary phase for capillary gas chromatography (GC) using trimethylsilyl derivatives. Notable differences in selectivity could be obtained by modifying the stationary or mobile phases. Separations of oxysterol pairs isomeric at side-chain carbons or C-7 were achieved on normal-phase, reversed-phase, chiral, or silver-ion HPLC columns. Chromatographic behavior is also described for side-chain hexadeuterated and heptafluorinated oxysterols, which are useful as standards in isotope dilution analyses and autoxidation studies, respectively. The overall results are relevant to many problems of oxysterol analysis, including the initial separation of oxysterols from cholesterol, determination of highly polar and nonpolar oxysterols, separation of isomeric pairs, selection of derivatization conditions for GC analysis, and quantitation of the extent of cholesterol autoxidation." @default.
- W4254702679 created "2022-05-12" @default.
- W4254702679 date "1995-06-01" @default.
- W4254702679 modified "2023-09-30" @default.
- W4254702679 title "Removal of cholesterol derivatives from egg yolk" @default.
- W4254702679 doi "https://doi.org/10.1016/s0924-2244(00)89071-8" @default.
- W4254702679 hasPublicationYear "1995" @default.
- W4254702679 type Work @default.
- W4254702679 citedByCount "0" @default.
- W4254702679 crossrefType "journal-article" @default.
- W4254702679 hasConcept C123460561 @default.
- W4254702679 hasConcept C178790620 @default.
- W4254702679 hasConcept C179998833 @default.
- W4254702679 hasConcept C185592680 @default.
- W4254702679 hasConcept C190196608 @default.
- W4254702679 hasConcept C2776923573 @default.
- W4254702679 hasConcept C2778111914 @default.
- W4254702679 hasConcept C2778163477 @default.
- W4254702679 hasConcept C2780471494 @default.
- W4254702679 hasConcept C2781321622 @default.
- W4254702679 hasConcept C31903555 @default.
- W4254702679 hasConcept C37329643 @default.
- W4254702679 hasConcept C43617362 @default.
- W4254702679 hasConcept C55493867 @default.
- W4254702679 hasConceptScore W4254702679C123460561 @default.
- W4254702679 hasConceptScore W4254702679C178790620 @default.
- W4254702679 hasConceptScore W4254702679C179998833 @default.
- W4254702679 hasConceptScore W4254702679C185592680 @default.
- W4254702679 hasConceptScore W4254702679C190196608 @default.
- W4254702679 hasConceptScore W4254702679C2776923573 @default.
- W4254702679 hasConceptScore W4254702679C2778111914 @default.
- W4254702679 hasConceptScore W4254702679C2778163477 @default.
- W4254702679 hasConceptScore W4254702679C2780471494 @default.
- W4254702679 hasConceptScore W4254702679C2781321622 @default.
- W4254702679 hasConceptScore W4254702679C31903555 @default.
- W4254702679 hasConceptScore W4254702679C37329643 @default.
- W4254702679 hasConceptScore W4254702679C43617362 @default.
- W4254702679 hasConceptScore W4254702679C55493867 @default.
- W4254702679 hasIssue "6" @default.
- W4254702679 hasLocation W42547026791 @default.
- W4254702679 hasOpenAccess W4254702679 @default.
- W4254702679 hasPrimaryLocation W42547026791 @default.
- W4254702679 hasRelatedWork W1941719721 @default.
- W4254702679 hasRelatedWork W1985303486 @default.
- W4254702679 hasRelatedWork W1986395714 @default.
- W4254702679 hasRelatedWork W1992606523 @default.
- W4254702679 hasRelatedWork W1994939190 @default.
- W4254702679 hasRelatedWork W2003071488 @default.
- W4254702679 hasRelatedWork W2026165519 @default.
- W4254702679 hasRelatedWork W2035534024 @default.
- W4254702679 hasRelatedWork W2086107997 @default.
- W4254702679 hasRelatedWork W2496327682 @default.
- W4254702679 hasVolume "6" @default.
- W4254702679 isParatext "false" @default.
- W4254702679 isRetracted "false" @default.
- W4254702679 workType "article" @default.