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- W4254759311 abstract "SUMMARY Seventy-three samples of serum, from 69 horses and one zebra, were subjected to the Rose Bengal Plate, serum tube agglutination, complement fixation, and anti-equine globulin (Coombs') tests for brucellosis. Fifty-one of the samples, from 48 horses, were submitted by practising veterinary surgeons; of these, 22 samples were associated with clinical conditions which might have been due to brucellosis. Fourteen samples were from healthy horses known to have been in contact with infected cattle, and six were from horses which were known not to have been exposed to brucellosis. More reactions at accepted diagnostic levels were obtained to the anti-globulin and complement fixation tests than to the agglutination test. Two horses showed no reactions, other than inconclusive titres to the antiglobulin test, and these titres could have resulted from exposure to Brucella. Eight of nine positive Rose Bengal tests were confirmed by a reaction at diagnostic level in at least one of the other tests, but two sera showing a reaction at diagnostic level to the other tests gave a negative Rose Bengal result. RÉSUMÉ Soixante treize échantillons de sérum, provenant de 69 chevaux et d'un zebre ont été testés au Rose Bengale, par séro agglutination en tube, par fixation du complement et à l'aide du Test de COOMBS, vis à vis de la Brucellose. 51 de ces échantillons étaient fournis par des praticiens, et 22 d'entre eux étaient associés à des conditions cliniques qui pouvaient être brucelliques. 14 prélèvements provendient de chevaux sains en contact avec du bétail contaminé et 6 provenaient de chevaux présumés n'avoir jamais été en contact avec une source brucellique. Des réactions positives obtenues par le Test de COOMBS et par fixation du complément furent plus nombreuses que celles obtenues par séro agglutination. Deux chevaux ne montrèrent aucune réaction autre que celle, douteuse, au Test de COOMBS, et les taux ambigus obtenus pouvaient indiquer une exposition à Brucella. Huit des neuf tests positifs au Rose Bengale furent confirmés par au moins une réaction à un des autres tests mais deux sérums ayant montré une réaction positive aux autres tests donnèrent une réaction négative au Rose Bengale. ZUSAMMENFASSUNG 73 Serumproben von 69 Pferden und einem Zebra wurden mit verschiedenen Methoden zum Nachweis von Brucellose untersucht: Bengalrot-Platte; Serum-Agglutination, Komplementbindung und Coombstest. 51 dieser Proben von 48 Pferden wurden von praktizierenden Tierärzten zur Verfügung gestellt; davon betrafen 22 Proben Pferde mit Brucellose-verdachtigen Krankheitsbildern. 14 Proben stammten von gesunden Pferden, von denen ein Kontakt mit infizierten Rindern bekannt war und sechs Proben wurden von Pferden gewonnen, bei denen eine Exposition unwahrscheinlich war. Mit der Anti-globulin (Coombs) Methode und der Komplementbindung wurden zahlreichere positive Reaktionen festgestellt als mit der Agglutinationsprobe. Zwei Pferde zeigten keine anderen Reaktionen als diagnostisch unsichere Titer im Coombstest; diese Titer könnte das Resultat einer Exposition mit Brucella gewesen sein. Acht von neun positiven Bengalrottests wurden von diagnostisch sicheren Ergebnissen mit mindestens je einer weiteren Methode bestätigt, aber zwei Seren mit positiven Reaktionen mit den anderen Methoden ergaben negative Bengalrot-Resultate." @default.
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- W4254759311 date "1975-07-01" @default.
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- W4254759311 title "Some Serological Reactions to “Brucella” Antigen in the Horse" @default.
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- W4254759311 doi "https://doi.org/10.1111/j.2042-3306.1975.tb03251.x" @default.
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