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- W4255477911 abstract "A tissue inhibitor of metalloproteinases (TIMP)-1 was isolated from human polymorphonuclear leukocytes (PMNL) in a complex with latent 95-kDa gelatinase (matrixmetalloproteinase, MMP-9). It was separated from the enzyme by gel fitration in the presence of SDS. Using a competitive ELISA procedure, we determined that 10% of the isolated gelatinase was complexed with TIMP-1. The presence of the inhibitor in isolated PMNL could also be demonstrated by indirect immunofluorescence using a specific antibody against TIMP-1. Cellular mRNA was isolated from PMNL, which were highly purified by separation via formylMet-Leu-Pro-stimulated chemotactic migration in a Boyden chamber. Using reverse-transcription PCR and Nothern blotting, TIMP-1 mRNA was shown to be present in PMNL, suggesting that these cells are also capable of synthesizing TIMP-1." @default.
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- W4255477911 date "1995-08-01" @default.
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- W4255477911 title "Evidence for the Tissue Inhibitor of Metalloproteinases-1 (TIMP-1) in Human Polymorphonuclear Leukocytes" @default.
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- W4255477911 doi "https://doi.org/10.1111/j.1432-1033.1995.tb20752.x" @default.
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