Matches in SemOpenAlex for { <https://semopenalex.org/work/W4255548629> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W4255548629 endingPage "3996" @default.
- W4255548629 startingPage "3986" @default.
- W4255548629 abstract "The Rh D antigen is the most clinically important protein blood group antigen of the erythrocyte. It is expressed as a collection of at least 37 different epitopes. The external domains of the Rh D protein involved in epitope presentation have been predicted based on the analysis of variant Rh D protein structures inferred from their cDNA sequences and their D epitope expression. This analysis can never be absolute because (1) most partial D phenotypes involve multiple amino acid changes in the Rh D protein and (2) deficiency for 1 or more epitopes may be due to gross structural alteration in the variant Rh D protein structure. We report here the amino acid requirements for the majority of D epitopes. They have been defined by generating a series of novel Rh mutant constructs by mutagenesis using an Rh cE cDNA as template and mutagenic oligonucleotide primers. When transfected into K562 cells, the D epitope expression of the derived mutant clones was then assessed by flow cytometry. The introduction of 9 externally predicted Rh D-specific amino acids on the Rh cE protein was sufficient to express 80% of all tested D epitopes, whereas other clones expressed none. We concluded from our data that the D epitope expression is consistent with at least 6 different epitope clusters localized on external regions of the Rh D protein, most involving overlapping regions within external loops 3, 4, and 6." @default.
- W4255548629 created "2022-05-12" @default.
- W4255548629 creator A5019497844 @default.
- W4255548629 creator A5054852950 @default.
- W4255548629 creator A5062550334 @default.
- W4255548629 creator A5076647694 @default.
- W4255548629 creator A5091023128 @default.
- W4255548629 date "1999-12-15" @default.
- W4255548629 modified "2023-10-17" @default.
- W4255548629 title "Molecular Configuration of Rh D Epitopes as Defined by Site-Directed Mutagenesis and Expression of Mutant Rh Constructs in K562 Erythroleukemia Cells" @default.
- W4255548629 cites W1517161569 @default.
- W4255548629 cites W1852905216 @default.
- W4255548629 cites W1885906116 @default.
- W4255548629 cites W1973143771 @default.
- W4255548629 cites W197328586 @default.
- W4255548629 cites W1997816446 @default.
- W4255548629 cites W2004647290 @default.
- W4255548629 cites W2005215437 @default.
- W4255548629 cites W2005940891 @default.
- W4255548629 cites W2008355891 @default.
- W4255548629 cites W2029320373 @default.
- W4255548629 cites W2040487429 @default.
- W4255548629 cites W2051483207 @default.
- W4255548629 cites W2059326097 @default.
- W4255548629 cites W2059751076 @default.
- W4255548629 cites W206238437 @default.
- W4255548629 cites W2065335184 @default.
- W4255548629 cites W2069879805 @default.
- W4255548629 cites W2072247780 @default.
- W4255548629 cites W2075114065 @default.
- W4255548629 cites W2083445427 @default.
- W4255548629 cites W2083620165 @default.
- W4255548629 cites W2114587365 @default.
- W4255548629 cites W2115900051 @default.
- W4255548629 cites W212667147 @default.
- W4255548629 cites W2136931671 @default.
- W4255548629 cites W2276244334 @default.
- W4255548629 cites W2289833142 @default.
- W4255548629 cites W34365839 @default.
- W4255548629 cites W4235435114 @default.
- W4255548629 cites W4239642457 @default.
- W4255548629 cites W4250419803 @default.
- W4255548629 cites W82570989 @default.
- W4255548629 doi "https://doi.org/10.1182/blood.v94.12.3986.424k18_3986_3996" @default.
- W4255548629 hasPublicationYear "1999" @default.
- W4255548629 type Work @default.
- W4255548629 citedByCount "2" @default.
- W4255548629 countsByYear W42555486292013 @default.
- W4255548629 crossrefType "journal-article" @default.
- W4255548629 hasAuthorship W4255548629A5019497844 @default.
- W4255548629 hasAuthorship W4255548629A5054852950 @default.
- W4255548629 hasAuthorship W4255548629A5062550334 @default.
- W4255548629 hasAuthorship W4255548629A5076647694 @default.
- W4255548629 hasAuthorship W4255548629A5091023128 @default.
- W4255548629 hasConcept C104317684 @default.
- W4255548629 hasConcept C143065580 @default.
- W4255548629 hasConcept C147483822 @default.
- W4255548629 hasConcept C153911025 @default.
- W4255548629 hasConcept C16318435 @default.
- W4255548629 hasConcept C187882448 @default.
- W4255548629 hasConcept C195616568 @default.
- W4255548629 hasConcept C2779926675 @default.
- W4255548629 hasConcept C515207424 @default.
- W4255548629 hasConcept C54355233 @default.
- W4255548629 hasConcept C55493867 @default.
- W4255548629 hasConcept C78598654 @default.
- W4255548629 hasConcept C86803240 @default.
- W4255548629 hasConceptScore W4255548629C104317684 @default.
- W4255548629 hasConceptScore W4255548629C143065580 @default.
- W4255548629 hasConceptScore W4255548629C147483822 @default.
- W4255548629 hasConceptScore W4255548629C153911025 @default.
- W4255548629 hasConceptScore W4255548629C16318435 @default.
- W4255548629 hasConceptScore W4255548629C187882448 @default.
- W4255548629 hasConceptScore W4255548629C195616568 @default.
- W4255548629 hasConceptScore W4255548629C2779926675 @default.
- W4255548629 hasConceptScore W4255548629C515207424 @default.
- W4255548629 hasConceptScore W4255548629C54355233 @default.
- W4255548629 hasConceptScore W4255548629C55493867 @default.
- W4255548629 hasConceptScore W4255548629C78598654 @default.
- W4255548629 hasConceptScore W4255548629C86803240 @default.
- W4255548629 hasIssue "12" @default.
- W4255548629 hasLocation W42555486291 @default.
- W4255548629 hasOpenAccess W4255548629 @default.
- W4255548629 hasPrimaryLocation W42555486291 @default.
- W4255548629 hasRelatedWork W1991572753 @default.
- W4255548629 hasRelatedWork W1996102030 @default.
- W4255548629 hasRelatedWork W2003116223 @default.
- W4255548629 hasRelatedWork W2016943232 @default.
- W4255548629 hasRelatedWork W2029383042 @default.
- W4255548629 hasRelatedWork W2062700146 @default.
- W4255548629 hasRelatedWork W3096915742 @default.
- W4255548629 hasRelatedWork W3139969521 @default.
- W4255548629 hasRelatedWork W3145510187 @default.
- W4255548629 hasRelatedWork W4241031629 @default.
- W4255548629 hasVolume "94" @default.
- W4255548629 isParatext "false" @default.
- W4255548629 isRetracted "false" @default.
- W4255548629 workType "article" @default.