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• W4256104342 abstract "Phospholipid-metabolizing enzymes are important participants in neutrophil signal transduction pathways. The methods discussed herein describe assays for assessing the activities of phospholipase A2 (PLA2), phospholipase C (PLC), phospholipase D (PLD), and phosphoinositide 3-OH-kinase in intact neutrophils. PLA2 activity is measured as the release of radiolabeled arachidonic acid. PLC activity is measured as the accumulation of inositol 1,4,5-trisphosphate (IP3), a water-soluble product, using a commercially available radioreceptor assay kit. PLD activity is measured as the appearance of its radiolabeled products, phosphatidic acid and phosphatidylethanol. PI3-K activity is measured as the appearance of its radiolabeled product, phosphatidylinositol-3,4,5-trisphosphate." @default.
• W4256104342 created "2022-05-12" @default.
• W4256104342 creator A5016536314 @default.
• W4256104342 creator A5067670293 @default.
• W4256104342 date "2014-01-01" @default.
• W4256104342 modified "2023-09-25" @default.
• W4256104342 title "Measurement of Phospholipid Metabolism in Intact Neutrophils" @default.
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• W4256104342 doi "https://doi.org/10.1007/978-1-62703-845-4_7" @default.
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• W4256104342 hasPublicationYear "2014" @default.
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