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- W4256151401 abstract "This chapter describes the assay method, the purification procedure, and the properties of glyoxalase I from mouse liver. The method is based upon the increase in absorbance at 240 nm because of the formation of S-D-lactoylglutathione. The purification procedure involves column material preparation, crude preparation, ethylamine-Sepharose 4B hydrophobic chromatography, and S-octylglutathione-Sepharose 4B affinity chromatography. Glyoxalase I from mouse liver has a molecular weight of 43,000; it is a dimer and is composed of identical subunits of molecular weights approximating 21,500. The purified enzyme exhibits a specific activity of 944 IU per milligram of protein employing the routine enzyme assay system. When the enzyme is stored at –30°C in the presence of glutathione, no significant loss of activity is observed for at least six months. There have been reports on some glyoxalase I inhibitors that are not substrate analogs; both competitive and noncompetitive inhibitions have been observed." @default.
- W4256151401 created "2022-05-12" @default.
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- W4256151401 date "1982-01-01" @default.
- W4256151401 modified "2023-09-23" @default.
- W4256151401 title "[86] Glyoxalase I from mouse liver" @default.
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- W4256151401 doi "https://doi.org/10.1016/s0076-6879(82)90182-3" @default.
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