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- W4256256753 abstract "Background Strong and stable transgene expression is fundamental to the success of recombinant adenovirus vectors in human gene therapy. However, control of transgene expression is a complex process, involving both viral and cellular factors. In this study, the influence of the E4 adenoviral region on the activity of various promoters was investigated in vitro and in vivo. Methods Pairs of isogenic E1° and E1°E4° vectors were generated and compared. Levels of transgene expression were determined by Northern blot, ELISA and FACS analysis. Initiation of transcription was studied by nuclear run-on assays. Results Similar to the viral CMV and RSV promoters, the activity of the ubiquitous cellular PGK promoter required the presence of the E4 genes in vitro and in vivo. In contrast, transgene expression from selected liver- and tumor-specific promoters did not require E4 functions. Conclusion Together with the reported low liver toxicity of E1°E4° vectors, the independence of E4 of liver-specific promoters renders such vectors interesting alternatives to the use of gutless vectors. Copyright © 2000 John Wiley & Sons, Ltd." @default.
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- W4256256753 date "2000-11-01" @default.
- W4256256753 modified "2023-09-26" @default.
- W4256256753 title "Differential influence of the E4 adenoviral genes on viral and cellular promoters" @default.
- W4256256753 doi "https://doi.org/10.1002/1521-2254(200011/12)2:6<433::aid-jgm143>3.3.co;2-t" @default.
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