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- W4256279460 abstract "A peroxyoxalate chemiluminescence detection in high-performance liquid chromatography was examined for determination of long chain aliphatic aldehydes using 4-(N,N-dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole (DBD-H) as a fluorogenic labelling reagent. A mixture of 0.6 mM bis(2,4,6-trichlorophenyl)oxalate and 100 mM H2O2 in acetonitrile was used as a post-column chemiluminescent reagent. DBD-hydrazones of five long chain aliphatic aldehydes (C11–C18:1) were eluted and well separated within 16 min with a mobile phase of 5 mM imidazole in acetonitrile. The detection limits were 14–18 fmol at a signal-to-noise ratio (S/N) of 2. DBD-hydrazones of four aliphatic aldehydes (C6–C11) were also eluted and well separated within 30 min with a mobile phase of 5 mM imidazole buffer (pH 7.5)-acetonitrile (20/80, v/v). The detection limits were 86–152 fmol (S/N=2)." @default.
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- W4256279460 date "1996-03-01" @default.
- W4256279460 modified "2023-09-26" @default.
- W4256279460 title "High‐Performance Liquid Chromatography of Long Chain Aliphatic Aldehydes with Peroxyoxalate Chemiluminescence Detection Utilizing a Fluorogenic Reagent, 4-(N,N-Dimethylaminosulphonyl)-7-hydrazino-2,1,3-benzoxadiazole" @default.
- W4256279460 doi "https://doi.org/10.1002/(sici)1099-0801(199603)10:2<99::aid-bmc553>3.3.co;2-8" @default.
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