FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4256287079> ?p ?o ?g. }

• W4256287079 endingPage "240" @default.
• W4256287079 startingPage "235" @default.
• W4256287079 abstract "EDTA saturated with Ca(2+), Fe(3+) or Cu(2+) can induce parthenogenetic activation of pig oocytes at the germinal vesicle stage, whereas EDTA saturated with Zn(2+), which is unable to chelate Zn(2+), does not, indicating that chelation of Zn(2+) with EDTA saturated with Ca(2+) (Ca-EDTA) in maturing pig oocytes plays a pivotal role in the induction of parthenogenetic activation of oocytes. In the present study, the involvement of Zn(2+) chelation in the induction of parthenogenetic activation of pig oocytes at the germinal vesicle stage was confirmed first by examining the effects of concomitant addition of Zn(2+), Cu(2+) or Ni(2+) at various concentrations together with 1 mmol Ca-EDTA l(-1) to the maturation medium. The titration experiments revealed that the pronuclear formation induced by 1 mmol Ca-EDTA l(-1) was completely inhibited by the addition of > 30 micromol Zn(2+) l(-1) to the medium, but not by the addition of Cu(2+) and Ni(2+) at any concentration examined. Second, bovine and mouse oocytes at the germinal vesicle stage were cultured in medium with or without 1 mmol Ca-EDTA l(-1) for 48 h to examine the effects of Ca-EDTA treatment on these oocytes during maturation culture. Most (70-86%) of the bovine oocytes that underwent germinal vesicle breakdown matured to the MII stage via the MI phase, regardless of whether Ca-EDTA was present for the first 24 h of culture. However, 61% of oocytes that had been cultured with Ca-EDTA for 48 h formed a pronucleus without a second polar body, whereas oocytes cultured in the absence of Ca-EDTA were not observed to form a pronucleus at any time during culture. However, even when mouse oocytes at the germinal vesicle stage were cultured for up to 48 h in maturation medium containing Ca-EDTA, pronuclear formation was not observed. Finally, when bovine oocytes that had been cultured with 1 mmol Ca-EDTA l(-1) for 48 h from the germinal vesicle stage were cultured further in medium without Ca-EDTA that was supplemented with 5% fetal calf serum, only 26% of the oocytes developed to the cleaved stage, and none could develop further." @default.
• W4256287079 created "2022-05-12" @default.
• W4256287079 creator A5041609489 @default.
• W4256287079 date "2002-08-01" @default.
• W4256287079 modified "2023-09-26" @default.
• W4256287079 title "Effects of EDTA saturated with Ca2+ (Ca-EDTA) on pig, bovine and mouse oocytes at the germinal vesicle stage during maturation culture and the involvement of chelation of Zn2+ in pronuclear formation induction by Ca-EDTA" @default.
• W4256287079 doi "https://doi.org/10.1530/reprod/124.2.235" @default.
• W4256287079 hasPublicationYear "2002" @default.
• W4256287079 type Work @default.
• W4256287079 citedByCount "0" @default.
• W4256287079 crossrefType "journal-article" @default.
• W4256287079 hasAuthorship W4256287079A5041609489 @default.
• W4256287079 hasBestOaLocation W42562870791 @default.
• W4256287079 hasConcept C103725303 @default.
• W4256287079 hasConcept C104317684 @default.
• W4256287079 hasConcept C130316041 @default.
• W4256287079 hasConcept C148196450 @default.
• W4256287079 hasConcept C153911025 @default.
• W4256287079 hasConcept C16685009 @default.
• W4256287079 hasConcept C179104552 @default.
• W4256287079 hasConcept C185592680 @default.
• W4256287079 hasConcept C196843134 @default.
• W4256287079 hasConcept C197404232 @default.
• W4256287079 hasConcept C2994119143 @default.
• W4256287079 hasConcept C41625074 @default.
• W4256287079 hasConcept C55493867 @default.
• W4256287079 hasConcept C71924100 @default.
• W4256287079 hasConcept C86803240 @default.
• W4256287079 hasConcept C95444343 @default.
• W4256287079 hasConceptScore W4256287079C103725303 @default.
• W4256287079 hasConceptScore W4256287079C104317684 @default.
• W4256287079 hasConceptScore W4256287079C130316041 @default.
• W4256287079 hasConceptScore W4256287079C148196450 @default.
• W4256287079 hasConceptScore W4256287079C153911025 @default.
• W4256287079 hasConceptScore W4256287079C16685009 @default.
• W4256287079 hasConceptScore W4256287079C179104552 @default.
• W4256287079 hasConceptScore W4256287079C185592680 @default.
• W4256287079 hasConceptScore W4256287079C196843134 @default.
• W4256287079 hasConceptScore W4256287079C197404232 @default.
• W4256287079 hasConceptScore W4256287079C2994119143 @default.
• W4256287079 hasConceptScore W4256287079C41625074 @default.
• W4256287079 hasConceptScore W4256287079C55493867 @default.
• W4256287079 hasConceptScore W4256287079C71924100 @default.
• W4256287079 hasConceptScore W4256287079C86803240 @default.
• W4256287079 hasConceptScore W4256287079C95444343 @default.
• W4256287079 hasIssue "2" @default.
• W4256287079 hasLocation W42562870791 @default.
• W4256287079 hasOpenAccess W4256287079 @default.
• W4256287079 hasPrimaryLocation W42562870791 @default.
• W4256287079 hasRelatedWork W1984849482 @default.
• W4256287079 hasRelatedWork W1994898947 @default.
• W4256287079 hasRelatedWork W2047623407 @default.
• W4256287079 hasRelatedWork W2050249664 @default.
• W4256287079 hasRelatedWork W2068246248 @default.
• W4256287079 hasRelatedWork W2127539124 @default.
• W4256287079 hasRelatedWork W2351370501 @default.
• W4256287079 hasRelatedWork W2568343533 @default.
• W4256287079 hasRelatedWork W2994006585 @default.
• W4256287079 hasRelatedWork W4306381918 @default.
• W4256287079 hasVolume "124" @default.
• W4256287079 isParatext "false" @default.
• W4256287079 isRetracted "false" @default.
• W4256287079 workType "article" @default.