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- W4256335082 abstract "A simple, rapid, and reliable method for the determination of residual sulphonamide antibacterials (SAs) (sulfadiazine, sulfamerazine, sulfadimidine, sulfamethoxypiridazine, sulfisozole, sulfamonomethoxine, sulfamethoxazole, sulfisoxazole, sulfadimethoxine, and sulfaquinoxaline) in animal liver and kidney was developed using a combination of clean-up on a Bond Elut PSA cartridge and HPLC with UV detection. The SAs were extracted with ethyl acetate and then dissolved in 5 ml of 50 v/v% ethyl acetate-n-hexane after being evaporated to dryness. For clean-up of the crude sample, the resuspended extract was applied to a Bond Elut PAS (primary/secondary amine cartridge), and then SAs were eluted from the cartridge using 5 ml of 20 v/v% acetonitrile–0.05 M ammonium formate before being analysed by HPLC. Recoveries of the SAs at the levels of 0.5 and 0.1 μg/g were 70.8–98.2%, the rerative standard deviation were less than 7.0%, and the detection limits were 0.03 μg/g. The present analysis method of SAs in animal kidney and liver using HPLC with a clean-up procedure was demonstrated to be highly applicable to the direct LC–MS–MS analysis without any modification." @default.
- W4256335082 created "2022-05-12" @default.
- W4256335082 date "1993-04-01" @default.
- W4256335082 modified "2023-09-30" @default.
- W4256335082 title "Intrinsic laryngeal muscle regeneration following endotracheal intubation" @default.
- W4256335082 doi "https://doi.org/10.1016/0165-5876(93)90101-8" @default.
- W4256335082 hasPublicationYear "1993" @default.
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