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- W4256702308 abstract "Exploratory purifications and assays of fractions for release of growth hormone (GH) revealed two separable entities each of which unambiguously released GH by radioimmunoassay. They are provisionally designated factor A-GHRH and factor B-GHRH until they are chemically and biologically characterized. After initial steps of isolation from porcine hypothalami, factor B-GHRH was extensively purified by stepwise chromatography using Bio-Gel P-2, Sephadex LH-20, Sephadex G-25 with a partition system of acetic acid-butanol-pyridine, DEAE-Sephadex A-25, and Bio-Gel CM-2. Assays showed that certain fractions were active, in vitro, at levels of ca. 15 μg. Factor B-GHRH is inhibited by somatostatin." @default.
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- W4256702308 date "1974-09-01" @default.
- W4256702308 modified "2023-09-28" @default.
- W4256702308 title "Identification and purification of factor B-GHRH from hypothalami which releases growth hormone" @default.
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- W4256702308 doi "https://doi.org/10.1016/0006-291x(74)90284-8" @default.
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