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- W426802110 abstract "Objective To obtain coding sequences of E6/E7 from human papillomavirus(HPV) type 16 and analysis the variation distribution to develop a novel molecular diagnosis method for cervical cancer.Methods Flow-through hybridization and gene chips were applied in identification of HPV subtype and screen HPV type 16 positive samples from cervical epithelium samples.HPV type 16 E6/E7 coding sequences were amplified by PCR with the specific primers.The PCR products were purified and the target sequences were cloned into pMD18-T vectors and fragments were determined by sequencing analysis.Variation analyses were performed using align tools including BLAST.Results HPV type 16 positive samples were screened through flow-through hybridization from 500 cervical epithelium samples.Three of them were selected for E6/E7 coding sequence amplification.Each reaction produced one fragment with size between 750 bp and 1 000 bp.Three coding sequences of E6/E7 of HPV type 16 were cloned and sequenced.The 3 sequences had been submitted to GenBank,the assigned accession numbers are: EU869316,EU869317 and EU869318.By BLAST,all above 99% identities with corresponding coding region of PPH16 in GenBank.Among three sequences eight kinds variances and four of them would cause coding amino acid change.Conclusion E6/E7 coding sequences and their variation profiles of HPV type 16 from three women's cervical epithelium has been obtained.The sequences and interrelated analysis result will be helpful to the further research of HPV carcinogenesis mechanism and development of novel molecular detective methods." @default.
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- W426802110 date "2009-01-01" @default.
- W426802110 modified "2023-09-26" @default.
- W426802110 title "Variation of E6/E7 coding sequence derived from human papillomavirus type 16." @default.
- W426802110 hasPublicationYear "2009" @default.
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