FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W427867463> ?p ?o ?g. }

• W427867463 abstract "Our lab was interested in creating a reproducible mechanism for obtaining neural crest cells in culture from cultured hindbrain explants of zebrafish. We have characterized the population of cells migrating from the explants as neural crest using a genetic variant of zebrafish with a GFP tag SOX-10, as well as immunostaining using HNK-1, a known neural marker for neural crest cells. We have also devised a modified protocol of in-situ hybridization that can be used for cells in culture for determining specific genes cells express in culture. Based on previous studies by Bingham et al., hindbrain explants have been extensively studied for preservation of their native (endogenous) cellular environments as well as the behavior of individual cell types. Biopidy ceramide labeling of live embryos has been used to extensively monitor and record cell shapes and organization in the developing zebrafish neural tube (Cooper et al). Studies by Bingham et al. have shown that morphogenesis of the neural tube in the zebrafish hindbrain is unaffected by the explantation of the hindbrain into a culture medium. The expression of krox20 and hoxb1a, which are expressed in the developing embryo in the hindbrain in rhombomeres 3 and 5 and rhombomere 4 respectively have been found to be unaffected when the hindbrain is explanted into a culture media. It has also been shown that valine expressing neural crest cells migrate normally out of r6. These results demonstrate that morphogenesis and patterning of the explanted hindbrain occur normally following the explanation of the hindbrain into a culture media (Bingham et al). Our lab has carried out dissections to remove the hindbrain from the developing embryo 15-18 hours post fertilization. We have found that it is possible to dissect the hindbrain out of a developing embryo and have cells migrate from these explants. We have also shown that these cells express Sox10 and are stained when using an antibody for HNK-1, which is known to be a neural crest cell marker. Our lab has also developed a working protocol of in-situ hybridization on cells in culture for Caco 2 and HT-29 using RNA probes for human beta actin and Hox A10." @default.
• W427867463 created "2016-06-24" @default.
• W427867463 creator A5020452877 @default.
• W427867463 date "2010-01-01" @default.
• W427867463 modified "2023-09-27" @default.
• W427867463 title "CHARACTERIZATION OF MIGRATING NEURAL CREST CELLS FROM HINDBRAIN EXPLANTS IN ZEBRAFISH" @default.
• W427867463 cites W1493719121 @default.
• W427867463 cites W1645069327 @default.
• W427867463 cites W1900442171 @default.
• W427867463 cites W1992755875 @default.
• W427867463 cites W2020830179 @default.
• W427867463 cites W2048686037 @default.
• W427867463 cites W2049803922 @default.
• W427867463 cites W2053188090 @default.
• W427867463 cites W2081424815 @default.
• W427867463 cites W2082639937 @default.
• W427867463 cites W2086253706 @default.
• W427867463 cites W2092073964 @default.
• W427867463 cites W2095153889 @default.
• W427867463 cites W2116191709 @default.
• W427867463 cites W2126107580 @default.
• W427867463 cites W2141405756 @default.
• W427867463 cites W2146650331 @default.
• W427867463 cites W2156952955 @default.
• W427867463 cites W2170886788 @default.
• W427867463 hasPublicationYear "2010" @default.
• W427867463 type Work @default.
• W427867463 sameAs 427867463 @default.
• W427867463 citedByCount "0" @default.
• W427867463 crossrefType "dissertation" @default.
• W427867463 hasAuthorship W427867463A5020452877 @default.
• W427867463 hasConcept C104317684 @default.
• W427867463 hasConcept C105702510 @default.
• W427867463 hasConcept C111039654 @default.
• W427867463 hasConcept C126749454 @default.
• W427867463 hasConcept C136834591 @default.
• W427867463 hasConcept C150194340 @default.
• W427867463 hasConcept C169760540 @default.
• W427867463 hasConcept C196843134 @default.
• W427867463 hasConcept C197849852 @default.
• W427867463 hasConcept C200707615 @default.
• W427867463 hasConcept C2776878037 @default.
• W427867463 hasConcept C2777075493 @default.
• W427867463 hasConcept C2780594696 @default.
• W427867463 hasConcept C28328180 @default.
• W427867463 hasConcept C54355233 @default.
• W427867463 hasConcept C80261634 @default.
• W427867463 hasConcept C86803240 @default.
• W427867463 hasConcept C95444343 @default.
• W427867463 hasConceptScore W427867463C104317684 @default.
• W427867463 hasConceptScore W427867463C105702510 @default.
• W427867463 hasConceptScore W427867463C111039654 @default.
• W427867463 hasConceptScore W427867463C126749454 @default.
• W427867463 hasConceptScore W427867463C136834591 @default.
• W427867463 hasConceptScore W427867463C150194340 @default.
• W427867463 hasConceptScore W427867463C169760540 @default.
• W427867463 hasConceptScore W427867463C196843134 @default.
• W427867463 hasConceptScore W427867463C197849852 @default.
• W427867463 hasConceptScore W427867463C200707615 @default.
• W427867463 hasConceptScore W427867463C2776878037 @default.
• W427867463 hasConceptScore W427867463C2777075493 @default.
• W427867463 hasConceptScore W427867463C2780594696 @default.
• W427867463 hasConceptScore W427867463C28328180 @default.
• W427867463 hasConceptScore W427867463C54355233 @default.
• W427867463 hasConceptScore W427867463C80261634 @default.
• W427867463 hasConceptScore W427867463C86803240 @default.
• W427867463 hasConceptScore W427867463C95444343 @default.
• W427867463 hasLocation W4278674631 @default.
• W427867463 hasOpenAccess W427867463 @default.
• W427867463 hasPrimaryLocation W4278674631 @default.
• W427867463 hasRelatedWork W108504879 @default.
• W427867463 hasRelatedWork W1505379989 @default.
• W427867463 hasRelatedWork W1975780402 @default.
• W427867463 hasRelatedWork W1976484322 @default.
• W427867463 hasRelatedWork W1995305478 @default.
• W427867463 hasRelatedWork W2016507865 @default.
• W427867463 hasRelatedWork W2023879953 @default.
• W427867463 hasRelatedWork W2034684350 @default.
• W427867463 hasRelatedWork W2050307937 @default.
• W427867463 hasRelatedWork W2052310776 @default.
• W427867463 hasRelatedWork W2053404888 @default.
• W427867463 hasRelatedWork W2071770395 @default.
• W427867463 hasRelatedWork W2089600001 @default.
• W427867463 hasRelatedWork W2154478552 @default.
• W427867463 hasRelatedWork W2156659930 @default.
• W427867463 hasRelatedWork W2377882060 @default.
• W427867463 hasRelatedWork W2465368075 @default.
• W427867463 hasRelatedWork W2790448481 @default.
• W427867463 hasRelatedWork W2809650325 @default.
• W427867463 hasRelatedWork W3012181114 @default.
• W427867463 isParatext "false" @default.
• W427867463 isRetracted "false" @default.
• W427867463 magId "427867463" @default.
• W427867463 workType "dissertation" @default.