FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280490640> ?p ?o ?g. }

• W4280490640 abstract "Abstract Background Kawasaki disease (KD) is an acute systemic vasculitis primarily affecting infants and children with an unclear etiology. Coronary artery aneurysm (CAA) is a common manifestation in severe KD patents, which may lead to thrombotic cardiovascular events such as heart attack and stroke even years after onset. We and others have previously reported systemic platelet activation in KD patients. Thymic stromal lymphopoietin (TSLP) is a recently identified interleukin-7 (IL-7) like cytokine associated with promoting pathological inflammation and most notably, platelet activation. The present study is to investigate the role of TSLP in KD-associated thrombosis. Methods To discover potential proteins underlying platelet activation in KD, we conducted a protein chip assay of 34 cytokines and discovered a significant upregulation in thymus stromal lymphopoietin (TSLP). ELISA corroborated the upregulation of TSLP in another group of KD patients. Clinical samples (plasma and platelets) from KD patients and healthy controls were analyzed via flow cytometry, immunofluorescent confocal microscopy, western blot, immunoprecipitation and thrombosis assays to reveal the underlying mechanisms. Results Among the 34 cytokines, we discovered several were aberrantly expressed, nine of which were continuously elevated after IVIG treatment and maintained during the convalescence in KD patients compared to healthy controls, including TSLP. The upregulation of TSLP in KD patients was confirmed by ELISA, which showed a further increase of TSLP in convalescent patients complicated with thrombosis. The expression of TSLP receptors (TSLPRs) on platelets were also significantly upregulated in KD patients complicated with thrombosis. Platelet activation, apoptosis, and mitochondrial autophagy (mitophagy) were increased in KD patients, which increase was exacerbated in convalescent patients complicated with thrombosis. In vitro, TSLP induced platelet activation and platelet mitophagy in healthy blood donors as we observed in KD patients. TSLP, similar to mitophagy agonist CCCP, promoted thrombosis, which was attenuated by the mitophagy inhibitor Mdivi-1. Co-immunoprecipitation in TSLP-treated platelets revealed TSLPR bound to mitophagy regulators, Parkin and VDAC1, suggesting a potential novel TSLP-mediated platelet mitophagy pathway. Conclusions Our results demonstrate that TSLP induces platelet mitophagy via a novel TSLPR/Parkin/VDAC1 mitophagy pathway that promotes thrombosis in KD. These results suggest TSLP as a novel therapeutic target against KD-associated thrombosis." @default.
• W4280490640 created "2022-05-22" @default.
• W4280490640 creator A5004378469 @default.
• W4280490640 creator A5010157362 @default.
• W4280490640 creator A5011932743 @default.
• W4280490640 creator A5025227750 @default.
• W4280490640 creator A5050268815 @default.
• W4280490640 creator A5053877613 @default.
• W4280490640 creator A5056623822 @default.
• W4280490640 creator A5058853115 @default.
• W4280490640 creator A5059839130 @default.
• W4280490640 creator A5059969775 @default.
• W4280490640 creator A5066716873 @default.
• W4280490640 creator A5067279151 @default.
• W4280490640 creator A5067730992 @default.
• W4280490640 creator A5072242045 @default.
• W4280490640 creator A5078990698 @default.
• W4280490640 creator A5086670785 @default.
• W4280490640 date "2022-05-12" @default.
• W4280490640 modified "2023-09-29" @default.
• W4280490640 title "TSLP induces platelet mitophagy and promotes thrombosis in Kawasaki disease " @default.
• W4280490640 doi "https://doi.org/10.21203/rs.3.rs-1628519/v1" @default.
• W4280490640 hasPublicationYear "2022" @default.
• W4280490640 type Work @default.
• W4280490640 citedByCount "0" @default.
• W4280490640 crossrefType "posted-content" @default.
• W4280490640 hasAuthorship W4280490640A5004378469 @default.
• W4280490640 hasAuthorship W4280490640A5010157362 @default.
• W4280490640 hasAuthorship W4280490640A5011932743 @default.
• W4280490640 hasAuthorship W4280490640A5025227750 @default.
• W4280490640 hasAuthorship W4280490640A5050268815 @default.
• W4280490640 hasAuthorship W4280490640A5053877613 @default.
• W4280490640 hasAuthorship W4280490640A5056623822 @default.
• W4280490640 hasAuthorship W4280490640A5058853115 @default.
• W4280490640 hasAuthorship W4280490640A5059839130 @default.
• W4280490640 hasAuthorship W4280490640A5059969775 @default.
• W4280490640 hasAuthorship W4280490640A5066716873 @default.
• W4280490640 hasAuthorship W4280490640A5067279151 @default.
• W4280490640 hasAuthorship W4280490640A5067730992 @default.
• W4280490640 hasAuthorship W4280490640A5072242045 @default.
• W4280490640 hasAuthorship W4280490640A5078990698 @default.
• W4280490640 hasAuthorship W4280490640A5086670785 @default.
• W4280490640 hasBestOaLocation W42804906401 @default.
• W4280490640 hasConcept C104317684 @default.
• W4280490640 hasConcept C126322002 @default.
• W4280490640 hasConcept C127561419 @default.
• W4280490640 hasConcept C203014093 @default.
• W4280490640 hasConcept C2778070265 @default.
• W4280490640 hasConcept C2778690821 @default.
• W4280490640 hasConcept C2780868729 @default.
• W4280490640 hasConcept C3018697912 @default.
• W4280490640 hasConcept C553184892 @default.
• W4280490640 hasConcept C55493867 @default.
• W4280490640 hasConcept C71924100 @default.
• W4280490640 hasConcept C86803240 @default.
• W4280490640 hasConcept C89560881 @default.
• W4280490640 hasConceptScore W4280490640C104317684 @default.
• W4280490640 hasConceptScore W4280490640C126322002 @default.
• W4280490640 hasConceptScore W4280490640C127561419 @default.
• W4280490640 hasConceptScore W4280490640C203014093 @default.
• W4280490640 hasConceptScore W4280490640C2778070265 @default.
• W4280490640 hasConceptScore W4280490640C2778690821 @default.
• W4280490640 hasConceptScore W4280490640C2780868729 @default.
• W4280490640 hasConceptScore W4280490640C3018697912 @default.
• W4280490640 hasConceptScore W4280490640C553184892 @default.
• W4280490640 hasConceptScore W4280490640C55493867 @default.
• W4280490640 hasConceptScore W4280490640C71924100 @default.
• W4280490640 hasConceptScore W4280490640C86803240 @default.
• W4280490640 hasConceptScore W4280490640C89560881 @default.
• W4280490640 hasLocation W42804906401 @default.
• W4280490640 hasOpenAccess W4280490640 @default.
• W4280490640 hasPrimaryLocation W42804906401 @default.
• W4280490640 hasRelatedWork W1615966168 @default.
• W4280490640 hasRelatedWork W2073408300 @default.
• W4280490640 hasRelatedWork W2083700293 @default.
• W4280490640 hasRelatedWork W2085154152 @default.
• W4280490640 hasRelatedWork W2107947912 @default.
• W4280490640 hasRelatedWork W2120131335 @default.
• W4280490640 hasRelatedWork W2129894791 @default.
• W4280490640 hasRelatedWork W2240106681 @default.
• W4280490640 hasRelatedWork W2591764090 @default.
• W4280490640 hasRelatedWork W50997392 @default.
• W4280490640 isParatext "false" @default.
• W4280490640 isRetracted "false" @default.
• W4280490640 workType "article" @default.