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• W4280491257 endingPage "107087" @default.
• W4280491257 startingPage "107087" @default.
• W4280491257 abstract "• Different developmental stages of zebrafish imaging using swept-source optical coherence tomography angiography (SSOCT) reported. • Enhanced phase sensitive SSOCT system is developed. • Depth-resolved volumetric imaging for microstructure & microvasculature visualization was performed. • In vivo imaging of day-to-day larval morphological development. • Depth-resolved OCTA of zebrafish cardiovascular system is testified. • The SSOCT system has potential to image the different developmental aspects of animal models in vivo . SSOCT is fast enough to acquire dense scans with negligible motion artifacts. Zebrafish as vertebrate model has applications in the fields of embryology, oncology, toxicology, molecular genetics and drug discovery due to its high homology with the human genome. The zebrafish embryogenesis presents a propitious model for concurrent visualization of developmental stages in vivo owing to transparency and ex-utero development of the embryo. Developments in imaging tools and techniques focus on providing real-time quantitative imaging. The present work demonstrates depth-resolved imaging of developing zebrafish using swept-source optical coherence tomography (SSOCT). The SSOCT system provides an axial resolution of 4.5 µm and an imaging depth of 3.26 mm. The paper also presents imaging of the developing larval microvasculature using a 1 µm swept-source optical coherence tomography angiography (ssOCTA). A phase-contrast technique of optical coherence angiography calculates the phase differences between consecutive B-scans taken at single location. This technique detects motion induced by moving scatterers. We have also performed depth-resolved OCT angiography to resolve the minute vasculature better. The SSOCT system can capture the detailed microstructures and microvasculature of model organisms in-vivo." @default.
• W4280491257 created "2022-05-22" @default.
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• W4280491257 date "2022-09-01" @default.
• W4280491257 modified "2023-10-01" @default.
• W4280491257 title "An in-vivo depth-resolved imaging of developing zebrafish microstructure and microvasculature using swept-source optical coherence tomography angiography" @default.
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• W4280491257 doi "https://doi.org/10.1016/j.optlaseng.2022.107087" @default.
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