FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280492569> ?p ?o ?g. }

• W4280492569 abstract "Chikungunya virus (CHIKV) is a re-emerged mosquito-borne alphavirus that can cause musculoskeletal diseases, imposing a substantial threat to public health globally. High-affinity antibodies are need for diagnosis and treatment of CHIKV infections. As a potential diagnostic and therapeutic agent, the multivalent VHH antibodies is a promising tookit in nanomedicine. Here, we developed potent multivalent VHH antibodies from an alpaca naïve phage display library targeting the E2 glycoprotein of the CHIKV virus.In the present study, we generated 20 VHH antibodies using a naïve phage display library for binders to the CHIKV E2 glycoprotein. Of these, multivalent VHH antibodies Nb-2E8 and Nb-3C5 had specific high-affinity binding to E2 protein within the nanomolar range. The equilibrium dissociation constant (KD) was between 2.59-20.7 nM, which was 100-fold stronger than the monovalent antibodies' affinity. Moreover, epitope mapping showed that Nb-2E8 and Nb-3C5 recognized different linear epitopes located on the E2 glycoprotein domain C and A, respectively. A facile protocol of sandwich ELISA was established using BiNb-2E8 as a capture antibody and HRP-conjugated BiNb-3C5 as a detection antibody. A good linear correlation was achieved between the OD450 value and the E2 protein concentration in the 5-1000 ng/mL range (r = 0.9864, P < 0.0001), indicating its potential for quantitative detection of the E2 protein.Compared to monovalent antibodies, multivalent VHH antibodies Nb-2E8 and Nb-3C5 showed high affinity and are potential candidates for diagnostic applications to better detect CHIKV virions in sera." @default.
• W4280492569 created "2022-05-22" @default.
• W4280492569 creator A5005689510 @default.
• W4280492569 creator A5015273050 @default.
• W4280492569 creator A5018244700 @default.
• W4280492569 creator A5026882044 @default.
• W4280492569 creator A5034949735 @default.
• W4280492569 creator A5041295761 @default.
• W4280492569 creator A5048930443 @default.
• W4280492569 creator A5051585041 @default.
• W4280492569 creator A5058088565 @default.
• W4280492569 creator A5069739945 @default.
• W4280492569 creator A5086003163 @default.
• W4280492569 creator A5091776489 @default.
• W4280492569 date "2022-05-14" @default.
• W4280492569 modified "2023-10-16" @default.
• W4280492569 title "Highly potent multivalent VHH antibodies against Chikungunya isolated from an alpaca naïve phage display library" @default.
• W4280492569 cites W1542207885 @default.
• W4280492569 cites W1545235721 @default.
• W4280492569 cites W1969557290 @default.
• W4280492569 cites W1973324389 @default.
• W4280492569 cites W1978940629 @default.
• W4280492569 cites W1979010514 @default.
• W4280492569 cites W1990847378 @default.
• W4280492569 cites W1993575340 @default.
• W4280492569 cites W1993712001 @default.
• W4280492569 cites W2007527881 @default.
• W4280492569 cites W2016191281 @default.
• W4280492569 cites W2028868321 @default.
• W4280492569 cites W2033079214 @default.
• W4280492569 cites W2036726021 @default.
• W4280492569 cites W2058641079 @default.
• W4280492569 cites W2063289063 @default.
• W4280492569 cites W2072537268 @default.
• W4280492569 cites W2076354932 @default.
• W4280492569 cites W2080434522 @default.
• W4280492569 cites W2095553811 @default.
• W4280492569 cites W2127003764 @default.
• W4280492569 cites W2138880157 @default.
• W4280492569 cites W2141231462 @default.
• W4280492569 cites W2141918346 @default.
• W4280492569 cites W2167217912 @default.
• W4280492569 cites W2169610187 @default.
• W4280492569 cites W2190300929 @default.
• W4280492569 cites W2257974587 @default.
• W4280492569 cites W2318238011 @default.
• W4280492569 cites W2519952216 @default.
• W4280492569 cites W2753031783 @default.
• W4280492569 cites W2788753373 @default.
• W4280492569 cites W2789909816 @default.
• W4280492569 cites W2791678513 @default.
• W4280492569 cites W2803841447 @default.
• W4280492569 cites W2810329724 @default.
• W4280492569 cites W2901197532 @default.
• W4280492569 cites W2911914006 @default.
• W4280492569 cites W2980388115 @default.
• W4280492569 cites W3000704432 @default.
• W4280492569 cites W3007146810 @default.
• W4280492569 cites W3008447349 @default.
• W4280492569 cites W3016614733 @default.
• W4280492569 cites W3025905826 @default.
• W4280492569 cites W3041222256 @default.
• W4280492569 cites W3044281741 @default.
• W4280492569 cites W3083786399 @default.
• W4280492569 cites W3094615536 @default.
• W4280492569 cites W3097927321 @default.
• W4280492569 cites W3110175658 @default.
• W4280492569 cites W3110382087 @default.
• W4280492569 cites W3123836702 @default.
• W4280492569 cites W3133616244 @default.
• W4280492569 cites W3154275519 @default.
• W4280492569 doi "https://doi.org/10.1186/s12951-022-01417-6" @default.
• W4280492569 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35568912" @default.
• W4280492569 hasPublicationYear "2022" @default.
• W4280492569 type Work @default.
• W4280492569 citedByCount "5" @default.
• W4280492569 countsByYear W42804925692023 @default.
• W4280492569 crossrefType "journal-article" @default.
• W4280492569 hasAuthorship W4280492569A5005689510 @default.
• W4280492569 hasAuthorship W4280492569A5015273050 @default.
• W4280492569 hasAuthorship W4280492569A5018244700 @default.
• W4280492569 hasAuthorship W4280492569A5026882044 @default.
• W4280492569 hasAuthorship W4280492569A5034949735 @default.
• W4280492569 hasAuthorship W4280492569A5041295761 @default.
• W4280492569 hasAuthorship W4280492569A5048930443 @default.
• W4280492569 hasAuthorship W4280492569A5051585041 @default.
• W4280492569 hasAuthorship W4280492569A5058088565 @default.
• W4280492569 hasAuthorship W4280492569A5069739945 @default.
• W4280492569 hasAuthorship W4280492569A5086003163 @default.
• W4280492569 hasAuthorship W4280492569A5091776489 @default.
• W4280492569 hasBestOaLocation W42804925691 @default.
• W4280492569 hasConcept C104317684 @default.
• W4280492569 hasConcept C108625454 @default.
• W4280492569 hasConcept C153911025 @default.
• W4280492569 hasConcept C159047783 @default.
• W4280492569 hasConcept C159654299 @default.
• W4280492569 hasConcept C167625842 @default.
• W4280492569 hasConcept C185592680 @default.
• W4280492569 hasConcept C186268636 @default.
• W4280492569 hasConcept C195616568 @default.

• next