Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280493087> ?p ?o ?g. }
- W4280493087 abstract "Fruit and vegetable wastes create unhygienic conditions and pose a environmental pollution. The utilization of such wastes as carbon sources for production of enzyme with microbial intervention could be an ecofriendly and profitable approach, apart from diminishing the waste load. The present investigation focused on the feasibility of using mosambi (Citrus limetta) peel as substrate for multienzyme production (pectinase, cellulase and amylase) through microbial intervention. Fifteen fungi were isolated from organic waste and screened in vitro their potential of biodegradation of mosambi peel through enzymes production. The best performing isolate was selected and identified as Trichoderma asperellum NG-125 (accession number-MW287256). Conditions viz. temperature, pH, incubation time and nutrient addition were optimized for efficient enzymes production. The maximum enzyme activity (U ml-1 min-1) of pectinase (595.7 ± 2.47), cellulase (497.3 ± 2.06) and amylase (440.9 ± 1.44) were observed at pH 5.5, incubation temperature of 30 °C after 10 days of fermentation. Moreover, macro-nutrients such as ammonium sulfate (0.1%) and potassium-di-hydrogen-ortho-phosphate (0.01%) further also enhanced the production of enzymes. The SDS-PAGE analysis of purified pectinase, cellulase and amylase using showed molecular mass of 43, 66 and 33 kDa, respectively. The enzyme retention activity (ERA) of aforesaid enzymes was also tested with four different natural fiber matrices viz., bagasse, rice husk, paddy straw and wheat straw. Among these, the maximum ERA was observed on bagasse matrix (pectinase-56.35%, cellulose-77.68% and amylase 59.54%). Enzymatic juice clarification yield obtained with test enzyme was 75.8%, as compared to 80.5% of commercial enzyme. The result indicates that T. asperellum may be exploited as multifaceted biocatalysis." @default.
- W4280493087 created "2022-05-22" @default.
- W4280493087 creator A5040929291 @default.
- W4280493087 creator A5054612755 @default.
- W4280493087 creator A5069048514 @default.
- W4280493087 creator A5071796822 @default.
- W4280493087 creator A5077099925 @default.
- W4280493087 creator A5088788441 @default.
- W4280493087 date "2022-05-11" @default.
- W4280493087 modified "2023-09-27" @default.
- W4280493087 title "Microbial production of multienzyme preparation from mosambi peel using Trichoderma asperellum" @default.
- W4280493087 cites W1142982933 @default.
- W4280493087 cites W1590838754 @default.
- W4280493087 cites W1591529662 @default.
- W4280493087 cites W1911066826 @default.
- W4280493087 cites W1967603055 @default.
- W4280493087 cites W1968659604 @default.
- W4280493087 cites W1986835041 @default.
- W4280493087 cites W2009674410 @default.
- W4280493087 cites W2018289835 @default.
- W4280493087 cites W2054726348 @default.
- W4280493087 cites W2059036698 @default.
- W4280493087 cites W2087956219 @default.
- W4280493087 cites W2100837269 @default.
- W4280493087 cites W2127148531 @default.
- W4280493087 cites W2131184446 @default.
- W4280493087 cites W2132632499 @default.
- W4280493087 cites W2150287329 @default.
- W4280493087 cites W2295843451 @default.
- W4280493087 cites W2424528550 @default.
- W4280493087 cites W2622278194 @default.
- W4280493087 cites W2766385738 @default.
- W4280493087 cites W2781599067 @default.
- W4280493087 cites W2789440701 @default.
- W4280493087 cites W2792709906 @default.
- W4280493087 cites W2808200167 @default.
- W4280493087 cites W2898515061 @default.
- W4280493087 cites W2898680107 @default.
- W4280493087 cites W2990061706 @default.
- W4280493087 cites W2990436319 @default.
- W4280493087 cites W3016064172 @default.
- W4280493087 cites W3035129734 @default.
- W4280493087 cites W3042244010 @default.
- W4280493087 cites W3111347511 @default.
- W4280493087 cites W3113401927 @default.
- W4280493087 cites W3147100536 @default.
- W4280493087 doi "https://doi.org/10.1007/s00203-022-02913-x" @default.
- W4280493087 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35543769" @default.
- W4280493087 hasPublicationYear "2022" @default.
- W4280493087 type Work @default.
- W4280493087 citedByCount "2" @default.
- W4280493087 countsByYear W42804930872023 @default.
- W4280493087 crossrefType "journal-article" @default.
- W4280493087 hasAuthorship W4280493087A5040929291 @default.
- W4280493087 hasAuthorship W4280493087A5054612755 @default.
- W4280493087 hasAuthorship W4280493087A5069048514 @default.
- W4280493087 hasAuthorship W4280493087A5071796822 @default.
- W4280493087 hasAuthorship W4280493087A5077099925 @default.
- W4280493087 hasAuthorship W4280493087A5088788441 @default.
- W4280493087 hasBestOaLocation W42804930872 @default.
- W4280493087 hasConcept C100544194 @default.
- W4280493087 hasConcept C134643618 @default.
- W4280493087 hasConcept C141603559 @default.
- W4280493087 hasConcept C150903083 @default.
- W4280493087 hasConcept C170835558 @default.
- W4280493087 hasConcept C172854840 @default.
- W4280493087 hasConcept C181199279 @default.
- W4280493087 hasConcept C185592680 @default.
- W4280493087 hasConcept C2775976191 @default.
- W4280493087 hasConcept C2779251873 @default.
- W4280493087 hasConcept C2779942233 @default.
- W4280493087 hasConcept C31903555 @default.
- W4280493087 hasConcept C55493867 @default.
- W4280493087 hasConcept C86803240 @default.
- W4280493087 hasConceptScore W4280493087C100544194 @default.
- W4280493087 hasConceptScore W4280493087C134643618 @default.
- W4280493087 hasConceptScore W4280493087C141603559 @default.
- W4280493087 hasConceptScore W4280493087C150903083 @default.
- W4280493087 hasConceptScore W4280493087C170835558 @default.
- W4280493087 hasConceptScore W4280493087C172854840 @default.
- W4280493087 hasConceptScore W4280493087C181199279 @default.
- W4280493087 hasConceptScore W4280493087C185592680 @default.
- W4280493087 hasConceptScore W4280493087C2775976191 @default.
- W4280493087 hasConceptScore W4280493087C2779251873 @default.
- W4280493087 hasConceptScore W4280493087C2779942233 @default.
- W4280493087 hasConceptScore W4280493087C31903555 @default.
- W4280493087 hasConceptScore W4280493087C55493867 @default.
- W4280493087 hasConceptScore W4280493087C86803240 @default.
- W4280493087 hasIssue "6" @default.
- W4280493087 hasLocation W42804930871 @default.
- W4280493087 hasLocation W42804930872 @default.
- W4280493087 hasLocation W42804930873 @default.
- W4280493087 hasOpenAccess W4280493087 @default.
- W4280493087 hasPrimaryLocation W42804930871 @default.
- W4280493087 hasRelatedWork W1603211896 @default.
- W4280493087 hasRelatedWork W1974125695 @default.
- W4280493087 hasRelatedWork W2100473923 @default.
- W4280493087 hasRelatedWork W2379582800 @default.
- W4280493087 hasRelatedWork W4205574367 @default.
- W4280493087 hasRelatedWork W4213212366 @default.