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- W4280493359 abstract "ABSTRACT As a renewable and sustainable source for energy, environment, and biomedical applications, microalgae and microalgal biodiesel have attracted great attention. However, their applications are confined due to the cost-efficiency of microalgal mass production. One-step strategy and continuous culturing systems could be solutions. However, current studies for optimization throughout microalgae-based biofuel production pipelines are generally derived from the batch culture process. Better tools are needed to study algal growth kinetics in continuous systems. A microfluidics chemostatic bioreactor array was presented, providing low-adhesion cultivation for algae in the gas, nutrition, and temperature (GNT) well-controlled environment with high throughput. The chip wasused to mimic the continuous culture environment of bioreactors. It allowed simultaneously studying of 8×8 different chemostatic conditions on algal growth and oil production in parallel on a 7×7 cm 2 footprint. On-chip experiments of batch and continuous cultures of Chlorella. sp . were performed to study growth and lipid accumulation under different nitrogen concentrations. The results demonstrated that microalgal cultures can be regulated to grow and accumulate lipids concurrently, thus enhancing lipid productivity in one step. The developed on-chip culturing condition screening, which was more suitable for continuous bioreactor, was achieved at a half shorter time, 64-times higher throughput, and less reagent consumption. It could be used to establish chemostat cultures in continuous bioreactors which can dramatically accelerate the development of renewable and sustainable algal for CO 2 fixation and biosynthesis and related systems for advanced sustainable energy, food, pharmacy, and agriculture with enormous social and ecological benefits. TEASER Sustainable microfluidic bioreactor for 64 times higher-throughput screening CO 2 fixation and biomass and biodiesel production in microalgae." @default.
- W4280493359 created "2022-05-22" @default.
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- W4280493359 date "2022-05-08" @default.
- W4280493359 modified "2023-09-26" @default.
- W4280493359 title "Microfluidic chemostatic bioreactor for high-throughput screening and sustainable co-harvesting of biomass and biodiesel in microalgae" @default.
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- W4280493359 doi "https://doi.org/10.1101/2022.05.06.490980" @default.
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