FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280494099> ?p ?o ?g. }

• W4280494099 endingPage "2201" @default.
• W4280494099 startingPage "2193" @default.
• W4280494099 abstract "The reprogramming of the genetic code through the introduction of noncanonical amino acids (ncAAs) has enabled exciting advances in synthetic biology and peptide drug discovery. Ribosomes that function with high efficiency and fidelity are necessary for all of these efforts, but for challenging ncAAs, the competing processes of near-cognate readthrough and peptidyl-tRNA dropoff can be issues. Here we uncover the surprising extent of these competing pathways in the PURE translation system using mRNAs encoding peptides with affinity tags at the N- and C-termini. We also show that hyperaccurate or error restrictive ribosomes with mutations in ribosomal protein S12 lead to significant improvements in yield and fidelity in the context of both canonical AAs and a challenging α,α-disubstituted ncAA. Hyperaccurate ribosomes also improve yields for quadruplet codon readthrough for a tRNA containing an expanded anticodon stem-loop, although they are not able to eliminate triplet codon reading by this tRNA. The impressive improvements in fidelity and the simplicity of introducing this mutation alongside other efforts to engineer the translation apparatus make hyperaccurate ribosomes an important advance for synthetic biology." @default.
• W4280494099 created "2022-05-22" @default.
• W4280494099 creator A5028327153 @default.
• W4280494099 creator A5055446896 @default.
• W4280494099 creator A5086835858 @default.
• W4280494099 date "2022-05-12" @default.
• W4280494099 modified "2023-10-03" @default.
• W4280494099 title "Hyperaccurate Ribosomes for Improved Genetic Code Reprogramming" @default.
• W4280494099 cites W13194283 @default.
• W4280494099 cites W1526809170 @default.
• W4280494099 cites W1587481053 @default.
• W4280494099 cites W1986656413 @default.
• W4280494099 cites W1987749012 @default.
• W4280494099 cites W1989248286 @default.
• W4280494099 cites W1989760659 @default.
• W4280494099 cites W1994839146 @default.
• W4280494099 cites W1997736987 @default.
• W4280494099 cites W1998052426 @default.
• W4280494099 cites W2002336386 @default.
• W4280494099 cites W2016206195 @default.
• W4280494099 cites W2031291845 @default.
• W4280494099 cites W2051556659 @default.
• W4280494099 cites W2067013502 @default.
• W4280494099 cites W2074869565 @default.
• W4280494099 cites W2080453586 @default.
• W4280494099 cites W2087181770 @default.
• W4280494099 cites W2100631905 @default.
• W4280494099 cites W2106687445 @default.
• W4280494099 cites W2117449534 @default.
• W4280494099 cites W2126780661 @default.
• W4280494099 cites W2136256341 @default.
• W4280494099 cites W2145029585 @default.
• W4280494099 cites W2145583354 @default.
• W4280494099 cites W2163223478 @default.
• W4280494099 cites W2170243611 @default.
• W4280494099 cites W2288141281 @default.
• W4280494099 cites W2327759216 @default.
• W4280494099 cites W2594846953 @default.
• W4280494099 cites W2745768305 @default.
• W4280494099 cites W2770708148 @default.
• W4280494099 cites W2784576562 @default.
• W4280494099 cites W2805523777 @default.
• W4280494099 cites W2885069640 @default.
• W4280494099 cites W2960681060 @default.
• W4280494099 cites W2998731295 @default.
• W4280494099 cites W3032151504 @default.
• W4280494099 cites W3045402853 @default.
• W4280494099 cites W3090968113 @default.
• W4280494099 cites W3098098111 @default.
• W4280494099 cites W3111662732 @default.
• W4280494099 cites W3182048773 @default.
• W4280494099 cites W3195899639 @default.
• W4280494099 cites W3196622003 @default.
• W4280494099 cites W3199575259 @default.
• W4280494099 cites W3202524566 @default.
• W4280494099 cites W3212528046 @default.
• W4280494099 cites W4226282105 @default.
• W4280494099 cites W2017743129 @default.
• W4280494099 doi "https://doi.org/10.1021/acssynbio.2c00150" @default.
• W4280494099 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35549158" @default.
• W4280494099 hasPublicationYear "2022" @default.
• W4280494099 type Work @default.
• W4280494099 citedByCount "4" @default.
• W4280494099 countsByYear W42804940992022 @default.
• W4280494099 countsByYear W42804940992023 @default.
• W4280494099 crossrefType "journal-article" @default.
• W4280494099 hasAuthorship W4280494099A5028327153 @default.
• W4280494099 hasAuthorship W4280494099A5055446896 @default.
• W4280494099 hasAuthorship W4280494099A5086835858 @default.
• W4280494099 hasBestOaLocation W42804940992 @default.
• W4280494099 hasConcept C104317684 @default.
• W4280494099 hasConcept C105580179 @default.
• W4280494099 hasConcept C106774688 @default.
• W4280494099 hasConcept C10853874 @default.
• W4280494099 hasConcept C149364088 @default.
• W4280494099 hasConcept C151730666 @default.
• W4280494099 hasConcept C153957851 @default.
• W4280494099 hasConcept C191908910 @default.
• W4280494099 hasConcept C20580545 @default.
• W4280494099 hasConcept C2776459999 @default.
• W4280494099 hasConcept C2779343474 @default.
• W4280494099 hasConcept C3675279 @default.
• W4280494099 hasConcept C41008148 @default.
• W4280494099 hasConcept C515207424 @default.
• W4280494099 hasConcept C54355233 @default.
• W4280494099 hasConcept C67705224 @default.
• W4280494099 hasConcept C70721500 @default.
• W4280494099 hasConcept C76155785 @default.
• W4280494099 hasConcept C86803240 @default.
• W4280494099 hasConcept C88478588 @default.
• W4280494099 hasConceptScore W4280494099C104317684 @default.
• W4280494099 hasConceptScore W4280494099C105580179 @default.
• W4280494099 hasConceptScore W4280494099C106774688 @default.
• W4280494099 hasConceptScore W4280494099C10853874 @default.
• W4280494099 hasConceptScore W4280494099C149364088 @default.
• W4280494099 hasConceptScore W4280494099C151730666 @default.
• W4280494099 hasConceptScore W4280494099C153957851 @default.
• W4280494099 hasConceptScore W4280494099C191908910 @default.

• next