Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280494767> ?p ?o ?g. }
- W4280494767 abstract "Excessive neuroinflammation and neuronal loss contribute to mechanisms of spinal cord injury (SCI). Accumulating evidence has suggested that topoisomerase 1 (Top1) inhibition can suppress exacerbated immune responses and protect against lethal inflammation. Pyroptosis is a recently identified pro-inflammatory programmed mode of cell death. However, the effects and underlying mechanisms of Top1 inhibition in SCI remains unclear. Locomotor functional recovery in mice was evaluated through Basso Mouse Scale (BMS). Neuronal loss was evaluated by immunochemistry staining of NeuN. Pyroptosis was determined by immunofluorescence staining, western blot, flow cytometry, cell viability, and cytotoxicity assays. In the present study, we estimated the effects of chemical inhibition of Top1 in an SCI model. Administration of Top1 inhibitor camptothecin (CPT) to mice significantly improved locomotor functional recovery after SCI. Moreover, CPT reduced Top1 level, inhibited nucleotide-binding oligomerization domain-like receptor 3 (NLRP3) inflammasome activation and pyroptosis, attenuated proinflammatory cytokines levels, diminished the number of neutrophil and neuronal loss in mice. Furthermore, CPT in oxygen-glucose deprivation neurons down-regulated Top1 level, attenuated NLRP3 inflammasome activation, and suppressed pyroptosis and inflammatory response. Together, our findings indicate that inhibition of Top1 with CPT can inhibit pyroptosis, control neuroinflammation, and improve functional recovery after SCI." @default.
- W4280494767 created "2022-05-22" @default.
- W4280494767 creator A5002338347 @default.
- W4280494767 creator A5043493952 @default.
- W4280494767 creator A5062613722 @default.
- W4280494767 creator A5070859616 @default.
- W4280494767 date "2022-05-17" @default.
- W4280494767 modified "2023-09-26" @default.
- W4280494767 title "Topoisomerase 1 inhibition modulates pyroptosis to improve recovery after spinal cord injury" @default.
- W4280494767 cites W1886677556 @default.
- W4280494767 cites W1926414860 @default.
- W4280494767 cites W1983325630 @default.
- W4280494767 cites W1986832448 @default.
- W4280494767 cites W1998760894 @default.
- W4280494767 cites W2031084334 @default.
- W4280494767 cites W2040771492 @default.
- W4280494767 cites W2067637968 @default.
- W4280494767 cites W2090323634 @default.
- W4280494767 cites W2096769354 @default.
- W4280494767 cites W2108757438 @default.
- W4280494767 cites W2109929056 @default.
- W4280494767 cites W2120049814 @default.
- W4280494767 cites W2167407423 @default.
- W4280494767 cites W2281138150 @default.
- W4280494767 cites W2292691093 @default.
- W4280494767 cites W2343003620 @default.
- W4280494767 cites W2343768426 @default.
- W4280494767 cites W2347417603 @default.
- W4280494767 cites W2393553112 @default.
- W4280494767 cites W2395695426 @default.
- W4280494767 cites W2462773201 @default.
- W4280494767 cites W2484283047 @default.
- W4280494767 cites W2552941476 @default.
- W4280494767 cites W2565953158 @default.
- W4280494767 cites W2584238603 @default.
- W4280494767 cites W2600346023 @default.
- W4280494767 cites W2753288478 @default.
- W4280494767 cites W2761624970 @default.
- W4280494767 cites W2761951098 @default.
- W4280494767 cites W2767111854 @default.
- W4280494767 cites W2767429780 @default.
- W4280494767 cites W2784275106 @default.
- W4280494767 cites W2797873249 @default.
- W4280494767 cites W2802371149 @default.
- W4280494767 cites W2804820563 @default.
- W4280494767 cites W2806906155 @default.
- W4280494767 cites W2808342347 @default.
- W4280494767 cites W2888567790 @default.
- W4280494767 cites W2898171168 @default.
- W4280494767 cites W2900532701 @default.
- W4280494767 cites W2902103207 @default.
- W4280494767 cites W2906821945 @default.
- W4280494767 cites W2907424910 @default.
- W4280494767 cites W2907994340 @default.
- W4280494767 cites W2908349335 @default.
- W4280494767 cites W2920778523 @default.
- W4280494767 cites W2924294387 @default.
- W4280494767 cites W2930689895 @default.
- W4280494767 cites W2936110531 @default.
- W4280494767 cites W2945795749 @default.
- W4280494767 cites W2955398906 @default.
- W4280494767 cites W2964898673 @default.
- W4280494767 cites W2969266828 @default.
- W4280494767 cites W2976536712 @default.
- W4280494767 cites W2980654938 @default.
- W4280494767 cites W2985879784 @default.
- W4280494767 cites W2995981280 @default.
- W4280494767 cites W3045452192 @default.
- W4280494767 cites W3118248510 @default.
- W4280494767 cites W3128260099 @default.
- W4280494767 cites W3147047699 @default.
- W4280494767 cites W3158173697 @default.
- W4280494767 cites W3174591919 @default.
- W4280494767 cites W3207588791 @default.
- W4280494767 doi "https://doi.org/10.1096/fj.202100713rr" @default.
- W4280494767 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35579890" @default.
- W4280494767 hasPublicationYear "2022" @default.
- W4280494767 type Work @default.
- W4280494767 citedByCount "1" @default.
- W4280494767 countsByYear W42804947672023 @default.
- W4280494767 crossrefType "journal-article" @default.
- W4280494767 hasAuthorship W4280494767A5002338347 @default.
- W4280494767 hasAuthorship W4280494767A5043493952 @default.
- W4280494767 hasAuthorship W4280494767A5062613722 @default.
- W4280494767 hasAuthorship W4280494767A5070859616 @default.
- W4280494767 hasConcept C164027704 @default.
- W4280494767 hasConcept C169760540 @default.
- W4280494767 hasConcept C17172800 @default.
- W4280494767 hasConcept C185592680 @default.
- W4280494767 hasConcept C190283241 @default.
- W4280494767 hasConcept C203014093 @default.
- W4280494767 hasConcept C204232928 @default.
- W4280494767 hasConcept C2776914184 @default.
- W4280494767 hasConcept C2777209026 @default.
- W4280494767 hasConcept C2778334475 @default.
- W4280494767 hasConcept C2779830541 @default.
- W4280494767 hasConcept C2780775167 @default.
- W4280494767 hasConcept C2781100881 @default.
- W4280494767 hasConcept C31573885 @default.
- W4280494767 hasConcept C55493867 @default.