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- W4280496766 abstract "Tetratrichomonas gallinarum and Trichomonas gallinae are pathogenic avian parasites that infect a wide range of bird species. The pathologic potential of T. gallinarum is controversial, whereas T. gallinae causes disease in many avian species. Infections are often asymptomatic in doves and pigeons; thus, columbids are presumed to represent the natural hosts for trichomonads. The detection of T. gallinarum and T. gallinae is based on direct microscopic observation or a conventional PCR assay. Microscopy is not very sensitive, and identification of the trichomonads at the genus or species level is not possible. Conventional PCR assays have been developed primarily for phylogenetic studies, which detect a wide range of Trichomonas spp. but do not allow their differentiation. We developed a duplex real-time PCR (rtPCR) assay for the simultaneous detection and differentiation of T. gallinarum and T. gallinae. We found that the rtPCR assay detected 10 2 plasmid DNA copies of T. gallinarum and as few as 10 1 plasmid DNA copies of T. gallinae." @default.
- W4280496766 created "2022-05-22" @default.
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- W4280496766 date "2022-05-20" @default.
- W4280496766 modified "2023-10-16" @default.
- W4280496766 title "A new duplex real-time PCR for simultaneous detection and differentiation of <i>Tetratrichomonas gallinarum</i> and <i>Trichomonas gallinae</i>" @default.
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- W4280496766 doi "https://doi.org/10.1177/10406387221098069" @default.
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