Matches in SemOpenAlex for { <https://semopenalex.org/work/W4280496858> ?p ?o ?g. }
- W4280496858 abstract "Abstract Soluble aggregates of the microtubule-associated protein tau have been challenging to assemble and characterize, despite their important role in the development of tauopathies. We found that sequential hyperphosphorylation by protein kinase A in conjugation with either glycogen synthase kinase 3β or stress activated protein kinase 4 enabled recombinant wild-type tau of isoform 0N4R to spontaneously polymerize into small amorphous aggregates in vitro. We employed tandem mass spectrometry to determine the phosphorylation sites, high-resolution native mass spectrometry to measure the degree of phosphorylation, and super-resolution microscopy and electron microscopy to characterize the morphology of aggregates formed. Functionally, compared with the unmodified aggregates, which require heparin induction to assemble, these self-assembled hyperphosphorylated tau aggregates more efficiently disrupt membrane bilayers and induce Toll-like receptor 4-dependent responses in human macrophages. Together, our results demonstrate that hyperphosphorylated tau aggregates are potentially damaging to cells, suggesting a mechanism for how hyperphosphorylation could drive neuroinflammation in tauopathies." @default.
- W4280496858 created "2022-05-22" @default.
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- W4280496858 date "2022-05-16" @default.
- W4280496858 modified "2023-09-27" @default.
- W4280496858 title "Hyperphosphorylated tau self-assembles into amorphous aggregates eliciting TLR4-dependent responses" @default.
- W4280496858 cites W1192337111 @default.
- W4280496858 cites W1600934558 @default.
- W4280496858 cites W1601433695 @default.
- W4280496858 cites W1893669995 @default.
- W4280496858 cites W1965709917 @default.
- W4280496858 cites W1967285029 @default.
- W4280496858 cites W1968232509 @default.
- W4280496858 cites W1971356093 @default.
- W4280496858 cites W1974322112 @default.
- W4280496858 cites W1980311499 @default.
- W4280496858 cites W1987398476 @default.
- W4280496858 cites W1989906820 @default.
- W4280496858 cites W1994282166 @default.
- W4280496858 cites W1995202691 @default.
- W4280496858 cites W2002839290 @default.
- W4280496858 cites W2003589106 @default.
- W4280496858 cites W2003974486 @default.
- W4280496858 cites W2004410209 @default.
- W4280496858 cites W2018423808 @default.
- W4280496858 cites W2018733438 @default.
- W4280496858 cites W2023193162 @default.
- W4280496858 cites W2026380581 @default.
- W4280496858 cites W2047700939 @default.
- W4280496858 cites W2056812842 @default.
- W4280496858 cites W2058570072 @default.
- W4280496858 cites W2060458492 @default.
- W4280496858 cites W2061531041 @default.
- W4280496858 cites W2062932176 @default.
- W4280496858 cites W2065495428 @default.
- W4280496858 cites W2072275270 @default.
- W4280496858 cites W2078459630 @default.
- W4280496858 cites W2087745495 @default.
- W4280496858 cites W2088825615 @default.
- W4280496858 cites W2092704615 @default.
- W4280496858 cites W2094525223 @default.
- W4280496858 cites W2104034347 @default.
- W4280496858 cites W2108802551 @default.
- W4280496858 cites W2112081368 @default.
- W4280496858 cites W2112136536 @default.
- W4280496858 cites W2122807946 @default.
- W4280496858 cites W2126020723 @default.
- W4280496858 cites W2131750038 @default.
- W4280496858 cites W2134396482 @default.
- W4280496858 cites W2147934559 @default.
- W4280496858 cites W2155304500 @default.
- W4280496858 cites W2157972442 @default.
- W4280496858 cites W2158591358 @default.
- W4280496858 cites W2162826697 @default.
- W4280496858 cites W2185034389 @default.
- W4280496858 cites W2226005455 @default.
- W4280496858 cites W2334348545 @default.
- W4280496858 cites W2336978805 @default.
- W4280496858 cites W2611519591 @default.
- W4280496858 cites W2727929522 @default.
- W4280496858 cites W2771683711 @default.
- W4280496858 cites W2772111047 @default.
- W4280496858 cites W2782293325 @default.
- W4280496858 cites W2783882235 @default.
- W4280496858 cites W2786377846 @default.
- W4280496858 cites W2788115976 @default.
- W4280496858 cites W2788827529 @default.
- W4280496858 cites W2789946978 @default.
- W4280496858 cites W2795001636 @default.
- W4280496858 cites W2806823430 @default.
- W4280496858 cites W2883474184 @default.
- W4280496858 cites W2896630904 @default.
- W4280496858 cites W2900691417 @default.
- W4280496858 cites W2927667967 @default.
- W4280496858 cites W2943729898 @default.
- W4280496858 cites W2947519870 @default.
- W4280496858 cites W3002969891 @default.
- W4280496858 cites W3004500623 @default.
- W4280496858 cites W3008384114 @default.
- W4280496858 cites W3011374615 @default.
- W4280496858 cites W3094092984 @default.
- W4280496858 cites W3102014803 @default.
- W4280496858 cites W3102160053 @default.