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- W4280498422 abstract "Abstract KRAS mutation is responsible for 40–50% of colorectal cancers (CRCs). RNA-seq data and bioinformatics methods were used to analyze the transcriptional profiles of KRAS mutant (mtKRAS) in comparison with the wild-type (wtKRAS) cell lines, followed by in-silico and quantitative real-time PCR (qPCR) validations. Gene set enrichment analysis showed overrepresentation of KRAS signaling as an oncogenic signature in mtKRAS. Gene ontology and pathway analyses on 600 differentially-expressed genes (DEGs) indicated their major involvement in the cancer-associated signal transduction pathways. Significant hub genes were identified through analyzing PPI network, with the highest node degree for PTPRC. The evaluation of the interaction between co-expressed DEGs and lncRNAs revealed 12 differentially-expressed lncRNAs which potentially regulate the genes majorly enriched in Rap1 and RAS signaling pathways. The results of the qPCR showed the overexpression of PPARG and PTGS2, and downregulation of PTPRC in mtKRAS cells compared to the wtKRAS one, which confirming the outputs of RNA-seq analysis. Further, significant upregualtion of miR-23b was observed in wtKRAS cells. The comparison between the expression level of hub genes and TFs with expression data of CRC tissue samples deposited in TCGA databank confirmed them as distinct biomarkers for the discrimination of normal and tumor patient samples. Survival analysis revealed the significant prognostic value for some of the hub genes, TFs, and lncRNAs. The results of the present study can extend the vision on the molecular mechanisms involved in KRAS-driven CRC pathogenesis." @default.
- W4280498422 created "2022-05-22" @default.
- W4280498422 creator A5007490685 @default.
- W4280498422 creator A5024434307 @default.
- W4280498422 creator A5035211736 @default.
- W4280498422 date "2022-05-13" @default.
- W4280498422 modified "2023-10-18" @default.
- W4280498422 title "Differential expression analysis of genes and long non-coding RNAs associated with KRAS mutation in colorectal cancer cells" @default.
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- W4280498422 doi "https://doi.org/10.1038/s41598-022-11697-5" @default.
- W4280498422 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35562390" @default.
- W4280498422 hasPublicationYear "2022" @default.
- W4280498422 type Work @default.