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- W4280508723 abstract "Trophoblasts are essential in fetal-maternal interaction during pregnancy. The goal was to study HLA profiles of primary trophoblasts derived from placentas, and to investigate their usefulness in studying interaction with immune cells.After enzymatic digestion of first-trimester placental tissue from seven donors (6-9 weeks gestation) and trophoblast enrichment we cultured cytotrophoblasts (CTB) in stem cell medium. CTB were differentiated into EVT in a Matrigel-containing medium. A subset of CTB/EVT was profiled for microRNA levels. Expression of classical HLA molecules and of HLA-G was studied by flow cytometry, qPCR, and ELISA. Secondary trophoblast cell lines JAR and JEG-3 were studied as controls. Lymphocytes were investigated during co-culturing with EVT.The trophoblasts could be easily maintained for several passages, upregulated classical trophoblast markers (GATA3, TFAP2C, chromosome-19 microRNAs), and upon differentiation to EVT they were selective in expressing HLA-C. EVT showed increasing expression of total HLA-G, an increasing proportion of HLA-G1 over G2- and G3 isoforms, and elevated excretion of soluble HLA-G. These features were distinct from those of the secondary trophoblast cell lines. TNF-α and IL-8 represented the most abundantly secreted cytokines by CTB, but their levels were minimal in EVT cultures. As proof of principle, we showed that EVT affect lymphocytes in three-day co-cultures (n=4) by decreasing activation marker HLA-DR.We verified the possibility culturing trophoblasts from first-term placentas, and their capability of differentiating to HLA-G expressing EVT. This culture model better represents the in-vivo situation than previously studied secondary trophoblast cell lines and enables mechanistic studies of fetal-maternal interactions." @default.
- W4280508723 created "2022-05-22" @default.
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- W4280508723 date "2022-05-13" @default.
- W4280508723 modified "2023-09-26" @default.
- W4280508723 title "Primary Trophoblast Cultures: Characterization of HLA Profiles and Immune Cell Interactions" @default.
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- W4280508723 cites W1979008257 @default.
- W4280508723 cites W1979311844 @default.
- W4280508723 cites W1981697215 @default.
- W4280508723 cites W1987970245 @default.
- W4280508723 cites W2008814547 @default.
- W4280508723 cites W2008838051 @default.
- W4280508723 cites W2020806673 @default.
- W4280508723 cites W2022275876 @default.
- W4280508723 cites W2029089280 @default.
- W4280508723 cites W2031955281 @default.
- W4280508723 cites W2035298228 @default.
- W4280508723 cites W2038592187 @default.
- W4280508723 cites W2050525926 @default.
- W4280508723 cites W2056089399 @default.
- W4280508723 cites W2056537517 @default.
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- W4280508723 cites W2088141357 @default.
- W4280508723 cites W2090739631 @default.
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- W4280508723 cites W2171224841 @default.
- W4280508723 cites W2171391056 @default.
- W4280508723 cites W2173028045 @default.
- W4280508723 cites W2222113668 @default.
- W4280508723 cites W2265249939 @default.
- W4280508723 cites W2340348123 @default.
- W4280508723 cites W2510397185 @default.
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- W4280508723 cites W2887483703 @default.
- W4280508723 cites W2898759023 @default.
- W4280508723 cites W2903489697 @default.
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- W4280508723 doi "https://doi.org/10.3389/fimmu.2022.814019" @default.
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