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- W4280517077 abstract "Actinomycetes are versatile secondary metabolite producers with great application potential in industries. However, industrial strain engineering has long been limited by the inefficient and labor-consuming plate/flask-based screening process, resulting in an urgent need for product-driven high-throughput screening methods for actinomycetes. Here, we combine a whole-cell biosensor and microfluidic platform to establish the whole-cell biosensor and producer co-cultivation-based microfluidic platform for screening actinomycetes (WELCOME). In WELCOME, we develop an MphR-based Escherichia coli whole-cell biosensor sensitive to erythromycin and co-cultivate it with Saccharopolyspora erythraea in droplets for high-throughput screening. Using WELCOME, we successfully screen out six erythromycin hyper-producing S. erythraea strains starting from an already high-producing industrial strain within 3 months, and the best one represents a 50% improved yield. WELCOME completely circumvents a major problem of industrial actinomycetes, which is usually genetic-intractable, and this method will revolutionize the field of industrial actinomycete engineering." @default.
- W4280517077 created "2022-05-22" @default.
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- W4280517077 date "2022-05-13" @default.
- W4280517077 modified "2023-10-17" @default.
- W4280517077 title "Whole-Cell Biosensor and Producer Co-cultivation-Based Microfludic Platform for Screening <i>Saccharopolyspora erythraea</i> with Hyper Erythromycin Production" @default.
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- W4280517077 doi "https://doi.org/10.1021/acssynbio.2c00102" @default.
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